Study On The Adsorption Mechanism And Performance Of Fluorescent Small Molecules And Graphene Oxide

Although fluorescent probes are widely distributed in various fields,in the field of biochemical research,fluorescent probes generally have poor selectivity,low signal-to-noise ratio,poor cytotoxicity,and poor response.In order to solve such problems,some scholars proposed to prepare biosensors by combining nanomaterials and fluorescent probes.Among them,graphene oxide has good biocompatibility,adsorption and fluorescence quenching ability.Many papers have reported that graphene oxide adsorbs fluorescent proteins,and nucleic acid probes cannot detect nucleic acids,proteins and lysosomes.All of them have achieved many good detection results,but there are few reports on small molecule probes and graphene oxide.In comparison,small molecule probes have absolute advantages in types,numbers,and high spatial resolution,but the adsorption mechanism between graphene oxide and small molecule probes and the performance of small molecule probes are still unclear..Carry out the adsorption test with graphene oxide first,according to the adsorption test data and the possible adsorption mechanism of the structure of coumarin and BODIPY.Then,after the probe is adsorbed by graphene oxide,a selective test and a test related to the ability to respond to glutathione are performed.According to the test results,the reason why the addition of graphene oxide to the properties of the probe changes is caused to cause graphene oxide Further verification of the adsorption mechanism with alternative fluorescent small molecules.