Preparation And Sensing Properties Of Molecular Imprinted Polymers Via MVL ATRP Based On Nickel And Gold Nanoclusters

Molecular imprinted technology（MIT）has been used in a wide range of analysis of recognition,due to the advantages of high selectivity,high sensitivity,high stability and simple synthesis method.Metal-free visible-light induced atom transfer radical polymerization（MVL ATRP）is a new method to achieve“living”/controlled polymerization.It can precisely control the end group,composition,structure and molecular weight of polymer.This method overcomes the defect of traditional ATRP catalyst which is sensitive to air and has toxicity to protein.Most of all,visible light has several advantages such as low price,easy to obtained,environmentally benign and low energy consumption.The use of nickel and gold nanoclusters increases the electrode surface area and improves the electron transfer ability.Therefore,we combined MIT and MVL ATRP together to obtain a new kind of MIP on nickel and gold nanoclusters.The specific research contents are as follows:1.3D nickel and gold nanoclusters were firstly prepared on the surface of the Au electrode.Then,by using the method of MVL ATRP,molecularly imprinted polymer was synthesized with erythromycin（ERY）as template molecule,acrylamide（AM）as functional monomer,N,N’-Methylenebisacrylamide（MBA）as crosslinking agent and fluorescein（FL）as catalyst.The imprinted electrode was characterized by cyclic voltammetry（CV）,electrochemical impedance spectroscopy（EIS）,scanning electron microscope（SEM）and energy dispersive X-ray spectroscopy（EDS）.Erythromycin was analyzed by differential pulse voltammetry（DPV）under optimized conditions.The linear response range was 1.0×10^-8～1.0×10^-1 mg/L,the detection limit was 3.2×10^-9 mg/L（S/N=3）,and the correlation coefficient was 0.997.2.Fluorescently labeled mouse IgG was used instead of traditional fluorescein as the catalyst and template for MVL ATRP.The molecularly imprinted polymer was successfully prepared on the surface of 3D nickel and gold nanoclusters under the condition of aqueous phase.The imprinted electrode was examinated by CV,EIS,SEM,X-ray photoelectron spectroscopy（XPS）,and“on-off”experiment.Under optimal conditions,the IgG was detected by DPV.The linear response range was 1.0×10^-7～10 mg/L,the detection limit was2.2×10^-8 mg/L（S/N=3）,and the correlation coefficient was 0.996.