Quinolone Molecularly Imprinted Sensors Based On Nano Gold And Graphene Materials

Quinolones are a class of antimicrobials that can be used to treat lesions caused by bacterial infections.Quinolones have many properties,including its broad spectrum of antibacterial properties and fast absorption speed which make it an important antibiotic.Currently quinolones have been developed to the fourth generation and used in the central nervous system,skeletal system,skin and soft tissue infections.The treatment for urinary tract infections has gained people’s approval and is known as one of the best clinically effective antibiotics.However,if the use of antibiotics is unreasonable,quinolones will remain in the human body and animals,causing many side effects and drug resistance directly or indirectly.Based on this,people need to find a method for the quantitative detection of quinolones,which can accurately and efficiently detect the residual amount to ensure the health of people and the environment.Molecular imprinting is a new technology emerging in recent years and has been used for the detection of drug residues.This technology overcomes the shortcomings of other detection techniques,it has the advantages of low cost,without the need for complex pretreatment of the sample.In this paper,the composite materials of nano-gold and reduced graphene was used to modify the electrode and the recognition components were established by molecular imprinting technology for quantitative determination of quinolone residues in the actual sample,including ciprofloxacin hydrochloride,enrofloxacin and norfloxacin.This paper,based on previous experiements,modified the electrode and explored the most promising conditions for the electrode,mainly in the following three parts:（1）The preparation of ciprofloxacin hydrochloride molecularly imprinted sensor based on pyrrole and o-phenylendiamine compound monomer,ciprofloxacin hydrochloride as template molecule,nano-gold reduction of graphene composite material as the electrode material,by cyclic voltammetry in building a molecularly imprinted membrane.By optimizing the preparation conditions and selecting the adsorption and elution conditions,a sensitive ciprofloxacin molecularly imprinted sensor was prepared.The detection range of this ciprofloxacin sensor is 1.0×10^-8-1.0×10^-2 mol/L,and the minimum detection limit is7.41×10^-12 mol/L.The sensor has good stability,easy preparation conditions,wide detection range,low detection limit,and can be used for actual sample detection.（2）In order to prapared a enrofloxacin molecularly imprinted sensor,pyrrolene and o-phenylenediamine as a composite monomer,norfloxacin as a template molecule,nano-gold reduction of graphene as a modified material,prepared by electropolymerization norfloxacin molecular imprinting sensor.The performance of the sensor was tested by square-wave voltammetry.When the norfloxacin concentration in the range of 1.0×10^-9-1.0×10^-2 mol/L,the difference between the current and the concentration of norfloxacin solution The logarithm value showed a good linear relationship,the linear equation wasΔI=0.09734logc+1.29324,the lowest detection limit was 5.19×10^-14 mol/L.Tested and compared with unmodified electrode,this enrofloxacin molecularly imprinted sensor has the advantages of wide linear range,low detection limit,reduced elution time and adsorption time,and is more conducive to the use of norfloxacin molecular imprinting sensor.（3）The enrofloxacin molecular imprinted film was prepared by cyclic voltammetry,and the glassy carbon electrode was modified with nanogold reduced graphene.The enrofloxacin molecule was used as a template molecule,and pyrrolephthalamide was used as a composite monomer.The cyclic voltammetry preparation of enrofloxacin molecularly imprinted membrane.A series of conditions for the preparation of enrofloxacin molecularly imprinted membrane were optimized by square wave voltammetry using potassium ferricyanide as probe molecule.All aspects of the enrofloxacin molecularly imprinted sensor prepared under optimal conditions The results showed that in the detection range of enrofloxacin molecular imprinting sensor in the range of 1×10^-8-1×10^-2 mol/L,the linear equation isΔI=0.0812logc+1.1840 with a detection limit of 1.56×10^-11 mol/L.The performance of this sensor is good,and provides a new idea for detecting the content of enrofloxacin in real samples.