Research On The Sub-damage Law Of Shear Stress Of Red Blood Cells Based On Raman Spectroscopy

The current evaluation of artificial heart pump blood damage focuses only on completely ruptured red blood cells.In fact,a large proportion of red blood cells will produce irreversible sub-damage after mechanical shearing.However,there is not enough attention to sub-injury at present,there is no objective method to evaluate the degree of sub-injury,and the effect of sub-injury on the oxygen-carrying function of red blood cells is still unclear.Therefore,this study is based on Raman spectroscopy technology,from the perspective of oxygen-carrying ability to detect and analyze the degree of red blood cell sub-damage.The traditional method of evaluating hemolysis only pays attention to the deficiency of completely ruptured red blood cells,which provides a new technical means for evaluating blood damage of artificial heart pumps.In this study,confocal Raman spectroscopy was used to analyze the influence of shear force amplitude,shear frequency and other factors on the blood oxygen carrying capacity from the internal analysis of hemoglobin,and conduct an experimental study on the effect of artificial heart pump actual shear on red blood cell sub-damage,The actual test standard hemolysis index,to verify the feasibility of the Raman method of evaluating the sub-damage of red blood cell oxygen carrying capacity.It provides a reference for establishing a more comprehensive evaluation system of artificial heart pump blood damage.By comparing the Raman spectrum of red blood cells with the Raman spectrum of the hemoglobin standard sample,it is found that the red blood cell spectrum can completely reflect the internal conformation of hemoglobin.Raman evaluation of red blood cell oxygen carrying capacity direct collection experiment The test results are consistent with the oxygenation collection experiment,indicating that the red blood cells have been irreversibly sub-damaged under the action of mechanical damage,and the structure of hemoglobin has changed.Raman spectroscopy is collected directly on the red blood cell samples.That is to analyze the changes in the oxygen-carrying capacity of red blood cells.Through Raman spectroscopy detection and analysis experiment,I₁₆₃₈/I₁₅₄₉peak intensity ratio curve fitting and blood gas analysis show that with the increase of the shear force amplitude,the oxygen carrying capacity of red blood cells decreases,and the left spectrum line at 1376cm^-1It is obviously elevated,the peak intensity at1549cm^-1and 1604cm^-1increases,and the vibration band of the oxygen concentration marker band?10 at 1638cm^-1decreases.After peak intensity ratio fitting,there is a negative linear relationship between I₁₆₃₈/I₁₅₄₉and the shear force amplitude.With the increase of the shear frequency,the oxygen-carrying capacity of red blood cells decreases rapidly,and then stabilizes after decreasing to a certain extent.The left spectrum line at 1376cm^-1is obviously elevated,and the peaks at 1549cm^-1and1604cm^-1are strong.After increasing to a certain extent,it will become stable,and the oxygen concentration marker band?10 at 1638cm^-1will become stable after weakening to a certain extent.After peak intensity ratio fitting,I₁₆₃₈/I₁₅₄₉decrease with the increase of frequency,and become stable after decreasing to a certain extent.Compared with the results of continuous flow blood pump red blood cell damage evaluation experiment,compared with the overall trend,the results of Raman evaluation method and continuous flow blood pump red blood cell damage evaluation method are similar,indicating that the red blood cell Raman spectroscopy method can effectively evaluate the sub-damage of red blood cell oxygen carrying capacity,The method is simple and reliable to analyze the oxygen-carrying capacity of red blood cells from a microscopic and functional point of view.This topic is based on the confocal Raman spectroscopy to conduct a microscopic and functional analysis of the sub-damage of red blood cells carrying oxygen.It pays attention to the degree of sub-damage of unruptured red blood cells,and has certain reference significance for the study of artificial heart blood compatibility.As an auxiliary judgment of the blood compatibility of the artificial heart pump.