| Glutathione(GSH)exists in all animal cells.Normally exist in sulfolol reduction.It is the main non-protein cymbal compound in the cell.It is a very important substance in the human body.It can participate in the body to participate in the body A variety of metabolic conditions have an important role in maintaining the normal function of the human body.Since GSH is a small molecular substance,compared with SOD and other large-molecular substances can be easier to enter cells,GSH can effectively play a clear effect on the oxygen free radicals inside and outside the cell,and play a direct or indirect effect in many life activities.Including gene expression regulation,enzyme activity and metabolic regulation,immune function regulation,protecting cells from active oxygen or toxin destruction,and amino acid transfer,etc.,oxidative stress or authentic compounds can reduce the intracellular GSH content,or convert into sulfur sulfur Glutathione Disulfide(GSSG).Therefore,detecting the distribution and concentration of GSH in-cell is of great significance for disease diagnosis and drug transmission.The fluorescent imaging analysis method has good selective selective,high sensitivity,simple operation,can conduct real-time quantitative detection of analytics,no destructive detection,and can also highly detect biomolecules in the biological environment and have high space-time resolution.The advantages,so it has received widespread attention in recent years.Especially in biological systems,because physiological activity substances are generally low concentrations,high reaction activity,and short life.In order to meet these requirements,to accurately determine the concentration of these substances in the cells,and fluorescence based on organic reactions is based on organic reactions.The probe emerges.Therefore,the use of a reasonable organic method design synthetic fluorescence probe for biological detection is of great significance.Our main purpose is to design high selectivity and high sensitivity to quickly detect the fluorescent probe of the intracellular sulfurnol.This thesis is based on the changes in fluorescent spectral properties after the specific effect of fluorescent probe and sulfitol material.Detection.We have designed a series of fluorescent probes based on pheasine to quantitatively detect GSH in the cells.Such molecules have been synthesized by three-step reaction,and Michael bonus reactions occurred by cymbal and cyanide ethylene obtained 5 reactive sulfur sulfur Alcohol fluorescent probe molecules.It has a structural representation of the nuclear speculation,carbon spectrum,infrared spectrum,and high-definition mass spectrum.The experimental results show that the five fluorescent probe molecules only show high selectivity and high sensitivity to 1 pair of probe 1 pair of glutathione.As the concentration of glutathione increases,the fluorescent strength of the compound gradually weakened.Under the condition of room temperature and physiological pH value,probe 1 can quickly detect glutathione,10?M probe 1 and 5.0m M glutathione is less than 35 min.There is no interference for the measurement.The concentration and fluorescent intensity of glutathione in the range of 1.0×10-3-5.0×10-3M are in a good linear relationship.The detection limit of the method is 1.112?M.Cell imaging and toxicity experiments show that this probe has excellent biocompatibility,cytosocular permeability and low toxicity,which can be used to quickly detect the concentration of biosiol in the cells. |
PDF Full Text Request