Physiological And Proteomic Analysis Of Arabidopsis Thaliana Under Cold Stress With Different Light Quality

Cold stress is the main environmental factor affecting plant growth and development.Under the condition of cold stress,the activities of related enzymes may decrease,cell dehydration and other adverse phenomena may occur in plants,and the related proteins may be induced to express,so that plants can adapt to low temperature stress and improve their cold resistance,which is conducive to the breeding of cold tolerant varieties.Light is an important condition for plant growth.Different wavelengths of light quality have different biological effects on plant morphology,growth and development.Arabidopsis thaliana is a model plant for the study of plant molecular/biochemical research system.In this study,Arabidopsis thaliana was treated with different light quality cold stress to explore the protein changes and up-regulated protein types of various organelles under different light quality conditions,so as to provide the corresponding proteomics basis for ornamental plants to select low temperature tolerant varieties by regulating light quality.In this study,Arabidopsis thaliana was used as the experimental material,combined with the detection of main physiological indexes and LC-MS/MS mass spectrometry identification technology,and then the differential protein biological analysis method was used to determine the main metabolic pathways of differential protein enrichment,and analyze the response mechanism of proteomics of Arabidopsis thaliana to cold stress under different light quality.The main results are as follows:(1)The soluble sugar content of Arabidopsis thaliana under different light quality and low temperature stress was 2.93±0.32 ?g/mL under white light treatment,and 0.51±0.07 ?g/mL under normal control.The overall trend was: white light ? red light ? dark and blue light quality.The content of proline was 161.12±1.06 ?g/mL in red light,109.13±2.01 ?g/mL in white light,and the lowest in blue light and dark.The content of proline in Arabidopsis thaliana was 9.92±1.01 ?g/mL.(2)The functional division and location division showed that the three functional proteins with the most changes were protein function,energy function and metabolic function proteins.Among them,229 differential proteins accounted for 31%,143 energy functional proteins accounted for 19%,122 metabolic functional proteins accounted for 17%,etc.The main changes of differential proteins were in plastid and cytosol,accounting for 42.2% and 30.1%of differential proteins,respectively.(3)Under different light quality cold stress conditions,the up-regulated proteins related to defense function of Arabidopsis thaliana cells were mainly in red light quality and no light.Cor47 and other cold regulatory proteins can also achieve functional expression in the absence of light and cold stress.In red light quality,ERD10,erd14 and hspa1 s are affected by red light quality,which can maintain cell homeostasis and prevent cell dehydration caused by cold stress.(4)Through go and KEGG bioinformatics analysis,protein synthesis accounted for the largest proportion in the functional classification of differential proteins.After keggpathway annotation,653 differential proteins were successfully annotated into 10 major metabolic pathways.The up regulation of S6 e,L12e,Se25 and other proteins in the red light quality conditional ribosome pathway can promote plant growth rate and alleviate plant senescence and death under cold stress.Under the condition of blue light quality cold stress,the photosynthetic pathway Pete,psag,PSAN,PSAF and other down regulated proteins weaken the light capture ability and electron transfer ability of Arabidopsis PS I,resulting in the Calvin cycle unable to obtain enough NADPH for carbon assimilation,resulting in weak plant growth under the condition of blue light quality cold stress.