Regulation Of AtWRKY7 Gene On Leaf Development

WRKY transcription factors is one of the largest transcription factor family in plants,and it plays an important role in plant stress,growth and development,secondary metabolism and hormone signal transduction.Leaves are the main organs of plants for photosynthesis,as well as essential vegetative organs of plants,and play a very important role in the life activities of plants.In this study,Arabidopsis thaliana was used as the research material to explore the biological function and mechanism of AtWRKY7.The main results are sumarized below:1.The c DNA and the promoter of the AtWRKY7 were cloned.The CDS has a full length of 1062 bp,encodes 354 aa,and contains two conserved domains: WRKY and Plant?zn?clust respecrively.Histochemical staining analysis shows that the AtWRKY7 is expressed in all tissues of Arabidopsis.The expression is most prominent at the leaf margin.2.AtWRKY7 overexpression homozygous lines have smaller leave,lobed leaf margin,stunted petal growth,thinner carpel,coampared with those of wild-type,and plant height also significantly lower than that of the wild-type,and with an accelerated aging phenotype.Under 400 m M mannitol and 42? heat shock treatment,the seeds of AtWRKY7 overexpression lines had a higher germination rate than that of the wild-type.3.Transcriptome analysis of wild-type,the atwrky7 mutant and AtWRKY7 overexpression lines showed that AtWRKY7 is involved in growth and development,senescence,hormone signal transduction and other regulatory processes.The above results indicate that AtWRKY7 participates in regulation of the developmental process of leaf size,lobed margin,stem elongation,petals and siliques development of A.Thaliana,and has a certain effect on senescence and abiotic stress tolerance.