Relationship Between Vitamin D Receptor Expression And Energy Metabolism In Human White Adipose Tissue

Purpose To study the relationship between vitamin D receptor and the expression of molecules related to energy metabolism in adipose tissue,and to explore the role of vitamin D receptor in energy metabolism in adipose tissue and energy balance in organism,so as to better understand the relationship between vitamin D and obesity.Method The subjects of our hospital from September 2018 to September 2019 were selected.According to the inclusion criteria,19 children who had undergone operations such as hernia and cryptorchidism were selected as research objects.Several abdominal subcutaneous white adipose tissues were collected during the operation,and the mRNA expression levels of vitamin D receptor,fatty acid synthase,Lipase(LIPE),carnitine palmitoyl transferase inhibitors-1(CPT1)and Uncoupling protein(UCPs)mRNA the white adipose tissues were detected by real-time RT-PCR.The relationship between VDR mRNA and FANS mRNA,LIPE mRNA,CPT1 mRNA and UCPs mRNA was analyzed.Result: The expression of VDR in human white adipose tissue has no correlation with the expression of lipid synthesis related gene FANS,lipid decomposition related gene LIPE and fatty acid ? oxidation related gene CPT1(FAS:r=-0.184,P>0.05;LIPE:r=0.096,P>0.05;CPT1:r=0.179,P>0.05);The expression of VDR in human white adipose tissue was positively correlated with the expression of UCP1 mRNA(r=0.896,P<0.01);However,VDR expression has no correlation with UCP2 mRNA and UCP3 mRNA expression(UCP2:r=-0.213,P>0.05;UCP3:r=0.271,P>0.05)?Conclusion: The expression of VDR is positively correlated with the expression of UCP1 mRNA in human white adipose tissue,which partly explains the positive correlation between vitamin d and basal metabolic rate.Vitamin D signal may play an important role in fat tissue energy metabolism and body energy balance.