| With the development of fluorescent dyes and optical imaging technology,fluorescent probe technology can perform in-situ visual detection of certain key signal molecules and mark-ers in life.Small-molecule fluorescent probes,as a detection tool with the advantages of easy synthesis and modification of structure,adjustable selectivity,high sensitivity,and non-inva-sive damage to cells and living bodies,have received extensive attention in the fields of chem-istry,biology,and medicine.In this paper,endogenous hydrogen peroxide and?-galactosidase,a tumor marker of ovarian cancer cells during the process of cellular oxygen-glucose stripping and reperfusion injury,were tested,and the following work was carried out:The H2O2 fluorescent probe with aggregation induced emission properties was designed and synthesized by using 4-vinylpyridinyl modified tetraphenylethylene as the fluorophore and phenylboronic acid as the H2O2 sensing group.The probe has good selectivity to hydrogen peroxide and is not affected by other interfering molecules;it has high sensitivity and a good linear relationship within the concentration range of hydrogen peroxide 75?250?M.It has a high sensitivity,and the minimum detection limit is 69 n M.The results of confocal imaging indicated that the probe was cell-permeable and capable of visualization of endogenous H2O2in OGD/R model He La cells and LPS-treated zebrafish.A near-infrared properties?-galactosidase fluorescent probe with immobilization function was designed and synthesized by using dicyanoisophorone derivative as the fluorophore,and the galactosyl group as the?-galactoside sensing group.UV-Vis and fluorescence emission spectrum test results show that the probe has good selectivity and high sensitivity to?-galacto-sidase,and the lowest detection limit is 1.185×10-3 U/m L.The results of confocal imaging indicate that the probe can be used for imaging studies to detect exogenous?-galactosidase liver cancer cells(HepG2). |
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