Construction Of A Novel Sulfur Dioxide/Hydrogen Sulfide /Formaldehyde Fluorescent Probe And Their Application In Bioimaging

Fluorescent probes have been widely used in life science,environmental science,materials science and other fields due to their advantages of simplicity,high sensitivity,high selectivity,low cost and real-time in situ detection.In this paper,naphthalimide-merocyanine,coumarin and naphthalimide were used as fluorophores to design and synthesize fluorescent probes to detection SO₂,H₂S and FA.The concrete contents are as follows:(1)We based on the Michael addition reaction mechanism,a fluorescent probe Lc-P with FRET structure was designed by using naphthalimide as donor,merocyanine as acceptor and piperazine as rigid structure.When SO₂ reacted with Lc-P,the FRET structure was destroyed,then the ratio detection of SO₂ was achieved.The spectrogram results showed that the probe Lc-P has good selectivity for SO₂ and could track SO₂ in vivo.(2)We adopted coumarin dye as fluorophore,which showed high fluorescence quantum yield,stable optical properties and large Stokes shift,and then designed a novel hydrogen sulfide probe La-P.The2,4-dinitrobenzenesulfonyl group was sulfurized by H₂S,which enhanced the fluorescence intensity.The probe La-P can detect exogenous and endogenous H₂S in living cells.(3)NaFP was designed based on the principle of hydrazine condensation.After FA reacted with NaFP,PET was destroyed and the fluorescence intensity was enhanced 565 times.The probe NaFP has high selectivity for detecting FA.NaFP showed that the concentration of formaldehyde changed under endoplasmic reticulum stress.In addition,the results of cell imaging experiments showed that NaFP could image exogenous and endogenous FA in living cell environment.