Study On The Toxicological Mechanism Of Disinfection By-products Halobenzoquinones In Drinking Water Using Glycoproteomics

Haloquinone is a new type of disinfection by-products,its toxicity can be more than1000 times of some regulated disinfection by-products.2,6-dichloro-1,4-benzoquinone(2,6-DCBQ)is a widely existed and relatively high halogenated benzoquinone disinfection by-product in tap water.The study on its distribution,toxicity and toxicological mechanism has attracted the attention of relevant departments and many researchers.Studies have shown that 2,6-DCBQ can cause serious cytotoxicity and organ toxicity.However,our understanding of its toxicity is still limited,and the relevant toxicological mechanism is not much studied.In this study,we use zebrafish as a biological model to analyze the toxic effects of2,6-DCBQ on heart tissue from different perspectives,and conducted a preliminary study on the toxicological mechanism based on the glycation proteomics method,providing some experimental basis for exploring the cell signaling pathways related to 2,6-DCBQ toxicity.The main research contents and results are as follows:(1)The heart,liver,spleen and gills of zebrafish were prepared to observe the organ toxicity of 2,6-DCBQ.The results showed that the liver,gills and heart of zebrafish were significantly affected by 2,6-DCBQ exposure.(2)Based on the above results,the toxicological mechanism of 2,6-DCBQ induced cardiotoxicity was further studied.By analyzing the expression level of sialic acid in zebrafish heart tissues,We found that with the increase of 2,6-DCBQ concentration,the relative area of Neu5 Ac and Neu5 Gc showed a trend of decreasing first and then increasing,which initially indicated that glycoprotein was associated with toxicity of 2,6-DCBQ(sialic acid in heart tissues mainly exists at the end of glycoprotein).(3)Based on the above experimental results,we use glycosylated proteomics methods to screen the glycosylated polypeptides related to 2,6-DCBQ exposure in zebrafish heart tissue.The results showed that the relative abundance of most of the identified glycoproteins showed an increasing trend and were mainly related to the process of glycolysis.This suggests that the toxicological mechanism of 2,6-DCBQ May involve its interference with the energy supply system of heart cells,which needs to be further explored.