Effect Of Branched Chain Amino Acids On Exercise Fatigue And Skeletal Muscle In Mice

Branched chain amino acid(BCAA)is a general term for Leu,Ile and Val,which are three essential branched chain amino acids.BCAA can be directly metabolized in muscle,participate in oxidative energy supply,synthesize protein,promote muscle growth and relieve exercise fatigue.At present,there are few studies on the microstructural changes of branched chain amino acids promoting skeletal muscle growth,side effects at high doses and the molecular mechanisms of fatigue related proteins.In order to explore the mechanism of feeding branched chain amino acids with different concentrations on promoting skeletal muscle growth and alleviating exercise fatigue in exercise training mice,KM mice in swimming exercise training were selected as the research subjects and randomly divided into two groups.Meanwhile,swimming exercise group was supplemented with different concentrations of BCAA.After swimming training for 50 days,the gastrocnemius muscle was sacrificed and dissected for morphological study,including conventional tissue structure,special structure and ultrastructure.Blood,liver,kidney and gastrocnemius muscles were collected for blood routine,blood muscle biochemical indexes and toxicity assessment of liver and kidney function.Finally take calves of IGF1,SIRT1 and COX? proteins and the positioning of quantitative research of the gene,and the final results indicate that:1.After adding BCAA movement has more weight/body weight ratio of the gastrocnemius muscle in mice and closer and thick muscle fibers,at the same time in the organization of the gastrocnemius muscle ? muscle fibers and elastic fibers increased,and BCAA concentrations were positively correlated,but the doses of more than 4 g/kg,d appears to slow or declining trend,ultrastructure of BCAA mice mitochondrial volume decreases,also found that mice fed BCAA sarcomere,muscle fiber diameter,muscle mass between small,transverse tubule diameter within the mitochondrial membrane and increase the thickness of the crest,But the change of BCAA concentration has little effect on it.2.Before the movement BCAA supplements may increase average red blood cell volume and the average red blood cell hemoglobin content,enhance the body's ability to carry oxygen,and significantly reduce the blood sugar,liver glycogen and muscle glycogen consumption,accelerate the metabolism of the lactic acid,but BCAA concentrations greater than 4 g/kg,d will result in a significant rise in levels of ALT and BUN,and significantly its mild kidney pathological cell damage phenomenon.3.The expression of IGF-1 protein in the gastrocnemius muscle of BCAA-fed exercise mice was increased,and the expression of protein gene was proportional to the concentration of BCAA.The protein was only located in the cytoplasm of the gastrocnemius muscle,but not in the nucleus.SIRT1 protein,strong expression in the cell nucleus,cytoplasm of expression but low intensity,SIRT1 protein and gene expression trend is consistent,both with BCAA concentrations is inversely proportional,but results in the highest dose group,in contrast,COX ? protein in are widely distributed in the muscle of calf muscles,and the distribution of protein is dot linear array,expressing quantity COX? with identical genes and BCAA concentrations higher than.Conclusions: Feeding BCAA can significantly increase skeletal muscle weight,promote skeletal muscle growth,enhance contractile efficiency and increase fatigue relief ability in exercise mice.Feasting BCAA could increase MCV and MCH contents in blood of exercise mice,reduce the consumption of BG,Mg and Hg,and accelerate LA metabolism.High concentration of BCAA had slight side effects on liver and kidney.BCAA can increase IGF 1and COX ? and reduce SIRT1 expression to enhance movement in skeletal muscle fatigue resistance and the effect of movement after skeletal muscle growth.