Studies On Auditory-visual Multisensory Perception And Auditory Decision-making Tasks Of Temporal Association Cortical Interneurons In Mice

Humans and animals feel the stimulation of the external environment via a variety of sensory organs.The brain can integrate these stimuli,so as to better perceive the environmental information and complete the cognitive tasks and behavioral choices accurately and efficiently.Our study has found that the temporal association cortex(Te A)of mice is with auditory-visual multisensory neurons in abundance,which is an important multisensory cortex.However,are there differences in the integration of multimodal sensory information between different types of interneurons(PV and SST)and pyramidal neurons(PNS)in the temporal association cortex? At present,there is no relevant research.Based on this,in the first part of this paper,we combined the method of buried multi-channel electrode recording and photogenetics to realize the accurate sorting of PV-INs and SST-INs in the temporal association cortex of mice.Objective to record the responses of neurons in the temporal association cortex of conscious mice to auditory,visual and auditory-visual multimodal stimuli,and to explore the similarities and differences of integrating multimodal information among PV-INs,SST-INs and pyramidal neurons in the temporal association cortex.In 7 PV-cre transgenic mice injected with p AOV-EF1a-DIO-h CHR2(H134R)-EYFP virus,549 neurons responding to sensory stimulation were recorded.According to the response to laser stimulation and spike waveform,47(8.56%)PV interneurons and 433(78.87%)pyramidal neurons were identified.In 6 wild-type C57BL/6 mice injected with p AAV-f SST-e Np HR3.0-HA-CW3 SL virus,a total of 472 neurons responding to sensory stimulation were recorded.According to the classification criteria,44(9.32%)were SST interneurons and 361(76.48%)were pyramidal neurons.Some PV interneurons,SST interneurons and pyramidal neurons showed the characteristics of multisensory enhancement.In-depth analysis showed PNs and SST-INs had stronger integration ability than PV-INs when the unimodal sensory response was weak,but there was no difference between them when the unimodal sensory response was strong.The results suggest that when the unimodal sensory response is weak,the integration ability of SST interneurons is stronger than that of PV interneurons,which can better regulate the firing response of other types of interneurons and pyramidal neurons,and the stronger integration ability of pyramidal neurons will help the brain to complete the corresponding task of integrating multisensory information.In real life,the object building perception through sensory information is rarely passive,and yet most of the perception will be associated with behavior.Decision making based on perceptual discrimination is the basic cognitive behavior of human and animals.In the second part of this paper,we focused on the response characteristics of PV interneurons,SST interneurons and pyramidal neurons in the temporal association cortex during auditory discrimination task.The results showed that the three kinds of neurons showed strong selectivity to 3k Hz and 12 k Hz sound stimulation when the mice learned the task,and the three kinds of neurons had similar characteristics.However,when the mice passively received sound stimulation before learning task,the firing responses of these three kinds of neurons to3 k Hz and 12 k Hz sound stimulation were similar,and there was no significant difference.The results of this study will deepen our understanding of the role of excitatory inhibitory circuits in the process of perception and cognition.