Active Behavioral States Laminar-specific Modulate The Neural Response In Mice Auditory Cortex

Information processing by the cerebral cortex and regulation of behavior state of consciousness, but this regulation in neural circuits is how little is known about the current implementation. In the visual and somatosensory nervous system, existing research shows physical activity in mice and beard swing states such acts can lead to cortical neurons membrane potential depolarization and neuronal firing desynchronization, these state changes of neurons changes in response to sensory stimulation level and stability. In the auditory nervous system, the different behavior of the auditory nerve in the information processing largely adjustment mechanism is not clear. Animal studies on the state of the nervous system to stimulate the auditory response adjustment mechanism is important for understanding the working mechanism of the auditory nervous system.In order to study the behavior of mice of different regulatory mechanisms auditory nervous system, we first developed a device to monitor the behavior of mice under the head fixed state. The system includes a rotatable includes flat plate, a speed measurement system and a video system. Based on this apparatus, according to the different state of the running speed of the head is fixed to the mice three different states are defined:stationary (V=0), the active non-runners (V<=1) and running (V>1). Secondly, we were recording the primary auditory cortex L1, L2/3, L4, MGBv auditory neurons in three different states in response to stimuli.Our results show that when the animal changes from the resting state to the active (including active non-running and running) state, for the same stimulation of sensory information in response to L2/3excitatory neurons is reduced, and the excitement of L4and MGBv responsive neurons remain constant.Existing research shows, L1median nerve auditory cortex million other sensory cortex received input projection. In order, to study the state of the animal L2/3neurons in response to auditory stimuli regulatory mechanisms, we further study the primary auditory cortex neurons in the auditory stimuli among different state response. Our results show that when the animal changes from the resting state to the active (including active non-running and running) state, for the same stimulation of sensory information in response to L1intermediate neurons are regulated. Therefore, the state animal response to auditory stimuli may regulate the system by regulating neuronal L1intermediate state to achieve.To further confirm whether the L1intermediate neurons regulate L2/3excitatory neurons play an important role in our L1to silence effect by neurons of the brain surface TTX add method, and study L2/3.and L4neurons in different states changes in auditory stimuli under the response. Our results show that, compared to before administration auditory stimulation L2/3excitatory neurons in response to administration significantly improved, and L4neurons stimulate the auditory response remains unchanged. Our data suggest that L1intermediate neurons can indeed adjust L2/3excitatory neurons.In summary, our data show that the different behavior of the primary auditory cortex of mice in response to adjustment of the stimulation effect of the different layers are different. When an animal changes from quiet to active auditory stimuli L2/3neurons response is reduced, while the neuronal pathways upload (MGBv and L4) in response to auditory stimuli remain unchanged. This adjustment may be through the regulation of neuronal intermediate L1to achieve.