Study On The Relationship Between Metabolomics Of Gastrocnemius In Rats And Anti-fatigue Ability

Anti-fatigue ability refers to the body's ability to delay the appearance of the body's maximum output power during exercise or consumption,that is,the ability to delay the occurrence of exercise fatigue.There has been a long history of research on fatigue,sports fatigue and anti-fatigue ability.There have been many research results since the 20 th century,but the reasons and mechanisms have not yet been fully explained.In recent years,with the rise of metabolomics research methods,a new idea has been provided for the detection and monitoring of exercise fatigue.This study is using metabonomics research methods to observe the relationship between the different metabolites related to the gastrocnemius muscle of rats with different anti-fatigue abilities and the relationship between anti-fatigue,find out the metabolites related to the anti-fatigue ability of rats,and try to further explore the effect of exercise fatigue The generation mechanism provides more references for the monitoring of sports training,the formulation of athletes' training plans and the selection of athletes.Objective: By observing the relationship between the different metabolites related to the gastrocnemius muscle of rats with different anti-fatigue abilities and anti-fatigue,find out the metabolites related to the anti-fatigue ability of rats,for the monitoring of sports training,the formulation of athletes' training plans and the selection of athletes Wait for more reference.Method: In this study,24 5-week-old male SD rats were selected as experimental subjects,weighing 250-350 g.All rats were fed with standard feed and freely eat and drink.After the rats were acclimatized in the animal room of this laboratory for 3 days,they were trained for 4 days of horizontal treadmill adaptation(gradient 0).Then select the speed of 25m/min(approximately 75% of the maximum oxygen uptake)to test the anti-fatigue ability of the rats.According to the results of the anti-fatigue ability test,the rats are divided into the strong anti-fatigue group(n=12)and the group with poor fatigue resistance(n=12).On the next day after the anti-fatigue ability test was completed,the rats were anesthetized with ether,and then sacrificed by cervical dislocation.The long and short heads of the gastrocnemius muscle were quickly separated with a glass minute needle to remove the long head of the left gastrocnemius in a refrigerator at-80 ?.Measurement.All samples were tested by ultra-high performance liquid chromatography and quadrupole time-of-flight mass spectrometer(UPLC-Q-TOF-MS).Results:(1)The group with strong anti-fatigue ability(12 rats with exerciseinduced fatigue appeared later),the time of appearance of fatigue was 26.72±8.14 minutes,which was significantly higher than that of the group with poor anti-fatigue ability 12 rats with exercise-induced fatigue appeared earlier)at 16.01±2.87 minutes,the difference was very significant(P<0.01).(2)There are obvious differences in the gastrocnemius metabolism profiles of rats with different anti-fatigue capabilities,and the differences are mainly caused by phase acid,prostaglandin E2,7,8-dimethyltetraoxazine and glutathione spermine.(3)Regarding the level of phase acid in the gastrocnemius muscle,the group with stronger anti-fatigue ability was significantly higher than the group with poor anti-fatigue ability,with significant difference(P<0.05).(4)Regarding the level of prostaglandin E2 in the gastrocnemius muscle,the group with stronger anti-fatigue ability was significantly higher than the group with poor anti-fatigue ability,and there was a significant difference(P<0.05).(5)For the level of 7,8-dimethyltetraoxazine in the gastrocnemius muscle,the group with stronger anti-fatigue ability was significantly higher than the group with poor antifatigue ability,with a significant difference(P<0.05).(6)Regarding the level of glutathione spermine in the gastrocnemius muscle,the group with stronger anti-fatigue ability was significantly higher than the group with poor anti-fatigue ability,with significant difference(P<0.05).Conclusion: This study found that the anti-fatigue ability of rats is closely related to the acid,prostaglandin E2,7,8-dimethyltetraoxazine and glutathione spermine in the gastrocnemius muscle.From the perspective of the metabolic pathways they reflect,Xanthine metabolism,arachidonic acid metabolism,riboflavin metabolism and glutathione metabolism may be important reasons affecting the anti-fatigue ability of rats.