Effects Of Inhibitor Of Nitric Oxide Synthesis On The Fighting Behavior Of Winged Dimorphic Velarifictorus Asperses

Fighting behavior exists widely in all kinds of animals.The fighting behavior of crickets is very typical among insects,and is one of the important model insects to study the regulation mechanism of insect fighting behavior at present.In our previous study,it was found that the fighting strategies of male adults of Velarifictorus asperses were different under different resource environments,that is,long-winged male adults had stronger fighting desire in fighting for cave territory,while short-winged male adults had higher winning probability in fighting for mate.In order to explore the neurophysiological regulation mechanism of the different fighting strategies of long-winged and short-winged male adults,this study was conducted by drug treatment.The effects of NG-Nitro-L-Arginine Methyl Ester(L-NAME),a synthetic nitric oxide(NO)inhibitor,on the fighting behavior of male adults of Velarifictorus asperses under different resource environments were investigated.The main results are as follows:1.When male adults were injected with 3 ?L L-NAME solution(1 mM)into their abdomen,there were no significant differences in fighting level,fighting duration and winning probability between treatment group and control group under the conditions of no cave and female,artificial cave and female presence.The results indicated that abdominal injection of NO synthesis inhibitor had NO significant effect on the fighting behavior of male adults of Velarifictorus asperses and could not effectively inhibit NO synthesis in brain nerve cells.2.When the male adult head was injected with 3 ?L L-NAME solution with a concentration of 1 mM,the fighting level and fighting duration of the male adults treated with drugs were significantly higher than that of the control group under the condition of no burrow and no female resources.When one of the fighting partners was treated with the drug,the victory rate of the treated male was significantly higher than that of the untreated male adults.It can be seen that injection of NO synthesis inhibitor into the head can promote the fighting behavior of male adults of Velarifictorus aspersus.3.In the presence of cave or female resources,male adults were injected with 3 ?L L-NAME solution at 1 mM on their heads,and there was no significant difference in fighting class and fighting duration between treatment group and control group.However,in the presence of cave,the winning probability of long-winged males was significantly lower than that of short-winged males.In the presence of female,the winning probability of short-winged males was significantly lower than that of long-winged males.These results indicated-that the effects of cave and female resources on the fight promotion of long-winged and short-winged male adults were related to the NO neural regulation pathway.Inhibition of NO synthesis would make it easier for the male adults of Velarifictorus aspersus to compromise in the fight for the above resources.These results indicated that NO was involved in the regulation of fighting behavior of male adults of Velarifictorus aspersus,and the regulatory effects varied under different resource and environmental conditions.This is related to the complexity and diversity of the neuroregulatory pathways of fighting behavior,and NO will activate different neuroregulatory pathways under different resource environments,leading to changes in different behavioral steps of fighting.