Comparison Of Main Energy Substances And Transcriptome Expression Differences In The Polymorphism Of Flight Behavior Of Female Adults Of Velarifictorus Aspersus

At present,the research on diffusion polymorphism mainly focuses on wing polymorphism,and there are very few studies on flight muscles and flight behavior polymorphism.The Velarifictorus aspersus has winged dimorphism.In previous studies,it was found that long-winged female and male adult worms have heritable polymorphism of flight behavior.Therefore,this study tested the long-winged female adults with different flight abilities in flight muscles and The differences in the main nutrients in the body are analyzed to analyze the metabolic basis of their flight behavior polytypes;transcriptome sequencing of female adults with different flight abilities will lay the foundation for further research on the molecular mechanisms of their flight behavior polytypes.The main results are as follows:(1)The main flight energy materials(triglycerides,protein,glycogen,total lipid content)of long-winged female adults with different flight abilities on the fifth day after emergence are significantly different.(2)The transcriptome sequencing of the female adults of the V.aspersus long-winged wing after flight,a total of 95.64Gb Clean Data was obtained,and each sample reached 6.02 Gb or more.Obtained 46404 transcripts,the highest length was 44099 bp,the average length was 1405.13 bp,and the CG content was 40.57%.After clustering and assembly analysis of the transcripts,a total of 36496 Unigenes were obtained,the highest length was 44099 bp,and the average length was 1411.02 bp.The CG content is 41.1%.(3)Comparing with NCBI protein database,Swiss-Prot protein database,KEGG database and COG database,a total of 16,584 Unigenes can be annotated with known biological functions,with an annotation rate of 45.33%,and annotated into the GO database 51 functional subcategories.After comparing with the Nr database and conducting homology analysis,the annotated species with the most matching sequence is the crypt termite Cryptotermes secundus,with a matching rate of 25.59%.(4)The transcriptome analysis of female adults with different flight abilities showed that 5650 differentially expressed genes were screened between long-winged female adults with weak flight ability and short-winged female adults,of which the number of up-regulated genes was 3178 and the number of down-regulated genes was 2472.873 differentially expressed genes were screened among long-winged female adults with weak and strong flying ability.Among them,the number of up-regulated genes was 492 and the number of down-regulated genes was 381.Differentially expressed genes were analyzed by GO enrichment.The differentially expressed genes between long-winged female adults with weak flight ability and short-winged female adults were mainly enriched in the function of cell components,and long-winged females with weak flight ability and strong long wings Differentially expressed genes among female adults are mainly enriched in molecular functions.The differentially expressed genes were analyzed by KEGG PATHWAY enrichment analysis.The differential genes between long-winged female adults and short-winged female adults with weak flight ability were mainly enriched in the large pathway of metabolism.Differentially expressed genes between long-winged female adults with weak and strong flying ability are also mainly enriched in the large pathway of metabolism.In this study,the transcriptome sequencing analysis of female adults of V aspersus was the first to provide a molecular basis for the study of cricket flight behavior polymorphism.