| Selenium can improve the human immunity,is a necessary element to eliminate HPV,threatening human health and life of more than 40 diseases are related to the lack of selenium.At present,the intake of selenium in many people is lower than the recommended intake.Selenium bioaugmentation is the most effective way to increase agricultural selenium concentration.Millet was the most important grain crop in ancient China,ranking the first among the five grains,and it was also the representative of the characteristic coarse grain industry in mountainous areas in the new era.The seeds are called "millet" after being shelled.Millet has attracted more and more attention from the public for its rich nutritional value and dietary efficacy.Studies have shown that selenium promotes the growth and development of food crops,enhances antioxidant properties,and causes structural changes in soil rhizosphere microbial communities.This study conducted field trials with millet as the material during 2020,setting four levels of control,low selenium,medium selenium,and high selenium(0,15 g·ha-1,30 g·ha-1,45 g·ha-1)(Na2SeO3),to study the changes in the microbial community diversity and community structure of the millet rhizosphere under different selenium levels,as well as the differences in plant agronomic traits,yield,nitrogen,phosphorus and selenium content and grain quality in millet root The correlation between the global micro-ecological environment and the above-ground physical and chemical properties provides the necessary scientific support for the production of selenium-rich millet with the best selenium concentration.The main conclusions are as follows:(1)The low and medium selenium levels changed the soil bacterial community structure without selenium treatment.Sucrase and pH significantly affected the changes of the rhizosphere soil bacterial and fungal community structure of Jigu 38,and urease significantly affected the changes of the rhizosphere soil fungal community structure of Jigu 19.In the rhizosphere soil communities of the two varieties,invertase and Actinomycota showed a significant positive correlation,and above-ground nitrogen had a significant positive correlation with Proteobacteria.(2)In the rhizosphere soil fungal community of Jigu 38,high Se level significantly decreased the relative abundance of Cephalopteris,but significantly increased the relative abundance of Cladospora.In the rhizosphere soil fungal community of Jigu 19,the relative abundance of Cephalopterygium and Cladospora were decreased by high selenium level,but the increase and decrease were not significant.The relative abundance of Basidiomycota and Acidobacteria were increased by selenium application.(3)At the jointing stage of millet growth,Se application could significantly increase the plant height of the two cultivars,and it increased with the increase of Se application level.At jointing stage and filling stage,medium selenium level had the best effect on chlorophyll content.Compared with any treatment,high selenium significantly inhibited the yield of millet.At the seedling,grain-filling and maturity stages,the activity of GSH-Px in the leaves of the two cultivars was significantly increased by medium and high Se levels.(4)Different concentrations of selenium fertilizer treatments significantly increased the selenium content of millet roots,stems and leaves,ears and grains.The medium selenium level has the best effect on increasing the selenium content of each part,and the high selenium level inhibits the increase of the selenium content of each part of millet.Under the same selenium level,the effect of improving the.selenium content of Jigu 38 is better than that of Jigu 19.Compared with the control group,the selenium level significantly increased the vitamin B1 content of the grains,and at the same time,it also had a tendency to increase the dietary fiber content of the grains.In conclusion,medium selenium level(30 g·ha-1)was the optimal fertilizer concentration for millet. |
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