Analyze And Research Speciation Of Selenium In Selenium-rich Plants

Selenium is one of the essential microelements of the human body; it is of greatimportance to health. This thesis is mainly about the quantitative analysis of Seleniumof different speciations in Selenium-rich plant samples, for the purpose of fullyunderstanding the distribution regulation of Selenium in Selenium-rich plant productsto provide reference for the production, development and usage of Selenium-richproducts.We made use of and improved the flow path of speciation-analysis of Se in plantsand the pre-transact method of samples according to references; used D101 big-boreabsorb resin instead of XAD-2 resin to eliminate the interference of dissolved organic;ascertained the concrete operating condition and flow path of water-extraction ofinorganic Se in plants; adopted first microwave-assisted digestion then wet-digestionmethod to transact samples, conspicuously improved the recovery of the method (up to97%); chose acid-deoxidization based on comparative experiments to deoxidize Se(Ⅵ)to Se(Ⅳ).The result of research showed that: the content percentage of inorganic Se,Se(Ⅳ),Se(Ⅵ) and dissolved Se and dissolved organic Se of garlics and spirulinatablets grown in selenium-rich environment is smaller (except organic Se andundissolved Se bigger)compared with the same kind common garlic and spirulinatablets. Se mainly exists in organic speciation, making up 93% or more of the totalamount. Among the inorganic speciation it mainly exists as Se(Ⅳ),the content of Se(Ⅵ)is smaller than 0.6% of the total amount; the amount of dissolved Se in the samples isabout 70%of the total amount of Se,the amount of dissolved organic Se makes upabout 60%～65% of the total amount of Se,and making up 90% or more of the totaldissolved Se; In terms of the enrich and invert of Se of the selenium-rich plants whichare grown in selenium-rich soil or under additional selenium culture, the Se from theenvironment is mainly inverted to organic Se. The accumulation of inorganic Se,especially Se (Ⅵ), is not conspicuous. Conclusively, the content of Se (Ⅵ) has noobvious tendency of increase with the enrichment of Se in the environment, anddifferent kind of plant's ability to enrich Se also differs. It fits in with the naturalregulation of plants caused by the differences of practical conditions such as varietiesand the environment.