Suppression Of Fusarium Wilt Of Banana By Different Pineapples-banana Rotation On Soil Microflora

Rotation of different crops is an important measure to overcome the obstacles of continuous cropping.Rotation between banana and pineapple can effectively alleviate the Fusarium wilt of banana.Therefore,this study used a combination of field research and pot experiment,using Real-time PCR and Miseq high-throughput sequencing method.Based on the technology to analyze the effects and mechanisms of different pineapple varieties in rotation.Moreover,analyzing the effects and mechanisms of pineapple residues to alleviate the biological barriers of the highly diseased banana soil.The main findings are as follows:The pot experiment showed that after planting “Golden Pineapple” and “Tai Nong 17”(B?GP and B?PP)in the banana garden soil with high blight,the number of culturable Fusarium oxysporum in the soil was significantly reduced,while increasing the soil organic matter and available phosphorus content.Real-time PCR showed that the number of Fusarium oxysporum in the rhizosphere of banana planting treatment(B)was higher than that of soil,while the number of Fusarium oxysporum in rhizosphere of different pineapple treatments was lower than that of soil;it was different from fallow(CK)and banana compared with the planting(B)treatment,the “Tai Nong 17” treatment(B?PP)significantly reduced the number of Fusarium oxysporum in the soil.At the same time,Miseq high-throughput sequencing results showed that the relative abundance of Fusarium grown in the “Tai Nong 17” planting treatment was significantly lower than other treatments.Moreover,after planting different pineapples,the Chao,ACE,Shannon and Invsimpson values of the soil bacteria and the microbial community structure were significantly changed,which also significantly increased the relative abundance of soil bacteria in GP1,Ktedonobacter,Penicillium,Mortierella,Talaromyces and Purpureocillium.Moreover,significantly increased the relative abundance of rhizobacteria Nitrolancea,Streptomyces,Talaromyces,Penicillium and Aspergillus.After “Golden Pineapple” and “Tai Nong 17” planted,the incidence of Fusarium wilt of replanted banana seedlings was significantly reduced.Compared with Z?CK treatment,the disease prevention rates were 79.17% and 88.43% respectively.Compared with the banana planting(Z?B)treatment,the disease prevention rates were 83.80% and 81.11%,respectively.After planting “Tai Nong 17”,the planting of banana seedlings increased significantly the absorption of nutrients in soil available phosphorus,available potassium,and alkali-decomposed nitrogen.The number of culturable Fusarium oxysporum and fungi after replanting bananas of different pineapples was significantly lower than that of banana continuous cropping treatment.Among them,the treatment of “Tai Nong 17”(Z?PP)significantly reduced the number of Fusarium oxysporum on the rhizosphere.The Real-time PCR results are consistent with this.Miseq high-throughput sequencing results also showed that the relative abundance of Fusarium oxysporum in soil treated with “Tai Nong 17”(Z?PP)was significantly lower than that of banana(Z?B).At the same time,the dominant acid bacilli Gp1,Talaromyces,Penicillium and Aspergillus,which existed after planting pineapple,were replanted bananas(Z?PP)after planting in “Tai Nong 17”.There is also significant presence in the soil.In addition,the treatment of “Tai Nong 17”(Z?PP)significantly increased the relative abundance of Burkholderia and Pseudomonas in the soil.The addition of 2% and 3% pineapple residues significantly reduced the occurrence of Fusarium wilt in the next crop of banana seedlings.Among them,the addition of 3%“Golden Pineapple”(C?GP)and “Tai nong 17”(C?PP)had better effects.Compared with no addition(C?CK),the disease prevention rates were: 55.17% and 65.52%;compared with adding 3% banana(C?B)residues,the disease prevention rates were: 63.89% and72.22%,respectively.At the same time,Real-time PCR results also showed that the number of Fusarium oxysporum in rhizosphere soil treated by “Tai Nong 17” was significantly lower than other treatments,and the relative abundance of Fusarium oxysporum in rhizosphere was consistent with it.Among them,the addition of 3% “Tai Nong 17” residue treatment significantly increased the bulk soil bacteria Bacillus,Gemmatimonas,Sphingomonas,and fungi Aspergillus,and Penicillium;Pseudomonas,Bacillus,Thauera,Elaphocordyceps and Aspergillus and Penicillium in the rhizosphere significantly increased bacteria.The addition of 1% and 10% of rhizome leaves and fruit extracts of different pineapple varieties can significantly inhibit the pathogen spore germination and mycelial growth;when 10% is added,it is more significant.14.59% and 21.88% respectively,while Balinese leaves,Balinese roots,Balinese fruits,Golden pineapple leaves,Golden pineapple roots,Golden pineapples,Tainong 17 leaves,Tainong 17 roots and Tainong 17 fruit against Acanthoporhis.The inhibition rates of Fusarium spore germination were 49.41%,63.76%,92.94%,48.47%,90.59%,92.71%,28.47%,51.29%,and 100.00%,among which the inhibitory effect of pineapple fruit was extremely obvious.To sum up,there are significant differences in the rotation of different pineapple varieties and their residues in relieving the banana continuous cropping obstacles.Among them,“Golden Pineapple” and “Tainong 17” have significant effects,and “Tainong 17”pineapple is the best.Real-time PCR and Miseq high-throughput sequencing showed that the “Tai Nong 17” rotation and its residues significantly reduced the number of Fusarium oxysporum in the soil,effectively controlled the occurrence of Fusarium wilt of banana seedlings,and significantly increased the soil bacteria Acidobacterium GP1,the relative abundance of Burkholderia and Pseudomonas,Penicillium,Talaromyces and Aspergillus among fungi.