The Screening Of Microbial Consortium With High Efficiency Cellulose Degradation And The Study Of Its Degradation Function

China is a developing country with agriculture as its primary industry.A large amount of straw resources are produced every year,but because of the underdevelopment of straw degradation technology,it is difficult to use,and the comprehensive utilization rate of straw is low.Degradation of straw by microorganisms can improve the degradation efficiency and help the efficient return of straw.However,the same strain has single type of enzyme,limited degradation ability,and undesirable degradation,so the research of microbial consortium is particularly important.In addition,the combination of biodegradation and chemical pretreatment can improve the utilization of straw resources.In order to obtain high-efficiency cellulose degradation microbial consortium and improve the degradation efficiency of the microbial consortium on straw,this study screened different bacterial strains from the corrupted straw and verified the function of microbial fertilizer.The effects of bacterial and fungal strains on the degradation of corn stover were studied,and the degradation conditions were optimized.The main findings are as follows:1.After preliminary screening by Congo red staining,the caboxy methyl cellulose enzyme activities and filter paper enzyme activities produced by the strain were used as the basis for further screening.A high-efficiency cellulose degradation bacterial microbial consortium composed of Pelomonas gx.+Curvibacter zj.and a high-efficiency cellulose degradation fungal microbial consortium composed of Trichoderma jw-1+Trichoderma jw-2 were obtained.The enzyme activity of microbial consortium is higher than that of the single strain.The enzyme activities of the bacterial microbial consortium is about 3.58 U/m L and 1.83 U/m L,the CMC and FPA activities of the fungal microbial consortium is about 2.76 U/m L and 1.52 U/m L.2.The enzyme-producing conditions of microbial consortium were studied.The results showed that the bacterial microbial consortium showed the maximum enzyme activity after incubation for 4 days at a temperature of 35 °C,a p H of 6.5 and the use of peptone as its sole nitrogen source.The fungal microbial consortium showed the maximum enzyme activity after 4 days of culture at a temperature of 30 °C,a p H of 6 and the use of peptone as its sole nitrogen source.3.The effects of the microbial consortium on the degradation efficiency of the pretreated straw were studied under the optimal conditions for enzyme production.The 40-mesh and 60-mesh corn granules were pretreated by different acid-base reagents,and the co-cultivation results showed that the microbial consortium combined with the 10% Na OH pretreatment process had the most degradation effect on corn stalks.The weight loss rate of straw was 78.1% after being degraded by bacterial microbial consortium,and the weight loss rate of straw was 76.8% after being degraded by fungal microbial consortium.In summary,a high-efficiency cellulose degradation bacterial microbial consortium and a high-efficiency cellulose degradation fungal microbial consortium were obtained.After pretreatment with 10% Na OH,the corn stalks were co-cultivated with microbial consortium under the optimal conditions of enzyme production,and both of them had higher degradation efficiency.The results provide a theoretical basis for the application of microorganisms with high efficiency in degrading cellulose.