Hypolipidemic Effect Of Bacillus Lactis DU-106 And Its Effect On Intestinal Flora Structure

With the change of people's dietary structure and the aggravation of population aging,hyperlipidemia has become an important factor inducing cardiac and cerebrovascular diseases.Studies have shown that hyperlipidemia is closely related to people's diet.One of the effective ways to intervene in hyperlipidemia is to adjust people's food structure.DU-106 is a newly discovered strain of Lactobacillus with good stability by Professor Du Bing of our university.In this paper,a mouse model of metabolic disorder was established by high-fat diet to study the effects of DU-106 diet on blood lipid metabolism:(plasma total triglyceride(TG),total cholesterol(TC),high density lipoprotein cholesterol(HDL-D),low density lipoprotein cholesterol(LDL-C)in rats.The lipid-lowering mechanism of DU-106 was explored at the molecular level by combining fecal metabonomics analysis technology with intestinal microbiology(Illumina sequencing technology).(1)By analyzing the plasma and the organ of rats,it was found that after 43 days of experiment,the liver weightindex of ratin the high-fat model group was significantly higher than that of the control group,while theliverweight index of DU-106 group was significantly lower than that of the high-fat model group.The contents of TC and LDL-C in hyperlipidemia model group were significantly higher than those in control group.The contents of TC and LDL-C in DU-106 intervention group were significantly lower than those in lipid model group,which improved lipid metabolism.It shows that DU-106 has the effect of lowering blood lipid.(2)LC-MS/MS technique was used to obtain the metabolic profile of rat feces.Combined with HMDB,KEGG and METPA database,it was found that the metabolic disorders in rats were mainly related to four metabolic pathways: glutamic acid metabolism,citrulline metabolism,lysine metabolism and aromatic compound metabolism.Cholic acid,deoxycholic acid,docosahexaenoic acid,L-aspartic acid,threoninemethionine,L-glutamic acid,L-(+)-citrulline and DL-lysinewere identified preliminarily and have 9 potential biomarkers.DU-106 can reduce blood lipid levels by affecting the above metabolism.(3)High throughput sequencing technology(Illumina sequencing technology)was used to study the microbiology of rat fecal samples.Alpha diversity analysis(OTU number,Shannon index,Chao1 index,ACE index)showed that the species richness of control group was higher than that of high fat group and intervention group.The diversity of microflora in the intervention group was higher than that in the lipid group,and the abundance of Coriobacteriia,Clostridia and Romboutsia increased.It can be concluded that this intervention can improve the diversity of microflora in the model rat.Beta diversity analysis(PCo A analysis,NMDS analysis,LEf Se analysis)showed that there were significant differences among the three groups of rat fecal samples(p < 0.05),and the difference between the intervention group and the control group was lower than that of the high-fat model group,so it can be judged that the intestinal flora disorder caused by high-fat can play a certain inhibitory role.In conclusion,Lactobacillus DU-106 can regulate the intestinal flora structure imbalance induced by high-fat diet in rat,which may be one of the important ways to intervene in the disorder of blood lipid metabolism caused by high-fat diet.