Effects Of Cypermethrin On Reproduction Development And Intestinal Flora Of Pubertal Male Rats

Pyrethroids,as environmental endocrine disruptors,can interfere with the binding of sex hormone receptors,and inhibit or stimulate the process of sex hormones' synthesis,release and,transportation.It will affect the normal function of the endocrine system,immune system,and reproductive system.With their increasing usage,their ecological health risks have received more attention.Recently,more and more studies showed that the intestinal flora,as a "super organ" in the human body,is closely related to various health problems.Moreover,the gut microbiota is affected by the combination of genes,food,exogenous compounds,and it also participates in various physiological,biochemical and metabolic activities of the human body.First,gut microbes can directly metabolize xenobiotics,or change their toxicity.Secondly,xenobiotics may also affect the host's health by changing the structure and composition of intestinal microorganisms,causing intestinal disorders.Therefore,researching the interaction between intestinal flora and metabolic mechanism,and its link between the host health,has an important significance in regulating the health risks of xenobiotics.In the early period of puberty,endocrine organs and morphological change very quickly.Because in this period,individual development is immature,and estrogen-like chemicals may cause persistent endocrine and reproductive organs of development irreversible injury or disability,and from the point of view of gametogenesis,males are more easily affected by estrogen-like compounds.Therefore,for estrogen-like compounds in male adolescent-this sensitive issue of the impact and role of the individual research is particularly important.We believe that when studying the effects of estrogen-like pesticides on male reproductive development and metabolism,the role of intestinal flora should not be ignored.Therefore,cypermethrin(CYP),which is a common compound,is applied to our animal experiments at 50,200 and 500 ug/kg.Using the male SD rats(PND 23)as the research model,the structure and function changes of intestinal microflora were analyzed by the method of microbial molecular ecology.The mechanism of the changes in intestinal microflora by pyrethroids and the metabolic disturbance on puberty reproductive development in male rats were analyzed and clarified.The main work and results are as follows:(1)After exposure to 500 ?g/kg cypermethrin,blood routine results showed that the red blood cell distribution width(RDW)of male rats increased significantly.The intestinal flora of male rats after exposure was analyzed by high-throughput sequencing with 16 S rRNA.The results showed a significant change in the intestinal flora of male rats of 500 ?g/kg cypermethrin.At the level of the family,the relative abundances of Bacteroidaceae,Veillonellaceae and Tannerellaceae increased significantly;at the genus level,the relative abundance of Prevotellaceae UCG-003 increased.Structural changes in the gut flora indicated that the male's immune balance may be disturbed.And since RDW is also associated with inflammation,we hypothesize that cypermethrin exposure may disturb the immune balance of pubertal males.(2)We found that the growth rate of male rats decreased after exposure to 500 ?g/kg cypermethrin,and the abundance of weight-related Prevotellaceae UCG-003 in the intestinal flora was significantly correlated,indicating that the intestinal flora imbalance after cypermethrin exposure resulted in Male rats have abnormal metabolism,which affects the weight gain of pubertal males.In summary,we studied the perturbation effect of cypermethrin on blood index,sperm index and intestinal flora in adolescent rats.We found that the intake of cypermethrin may increase the metabolic disorder risks in the body by changing the normal composition and structure of the intestinal flora.Health risks such as immune response suggest an important role for gut microbiota in the body and the urgency of incorporating gut microbes into environmental health risk assessment.Our research provides new models and ideas for the future study of the toxic effects of pyrethroid pesticides,and provides an important reference for the development of pesticides with low ecological risks.