The Study Of Gene Expression Profiling In The Process Of Satellite Glial Cells Transdifferentiation Into Neurons

Objective: By studying the gene expression profile of satellite glial cells transdifferentiated into neuron process,the differentially expressed m RNA was screened to find possible target genes,and the function of target genes was preliminarily verified.It is hoped to provide theoretical support for the possible regulatory mechanisms of satellite glial cell transdifferentiation.Methods: The second generation gene sequencing technology was used to sequence the induced satellite glial cells.The sequence results were screened by enrichment analysis of GO and KEGG,and the possible target genes were screened.The expression of target gene was verified by qRT-PCR,and the function of target gene was verified after knocking down.Results:1.The correlation between samples was over 0.8,and the results of qRT-PCR were consistent with those of sequencing.2.The results of GO and KEGG enrichment analysis show that there is "crosstalk" between Wnt signaling pathway and Neurotrophin signaling pathway.3.Protein-protein network interaction analysis of Wnt signaling pathway and Neurotrophin signaling pathway showed that Wnt4?FZD6?Ctnnb1?Igf1?Igf1r?Ngf and Ntrk3 are possible target genes.4.Ctnnb1 has the most significant difference in in vivo and in vitro experiments,p<0.001.5.After knocking down Ctnnb1,the degree of apoptosis was significantly reduced,and the expression of related factors was consistent with it in the process of satellite glial cell transdifferentiation.Conclusion:1.There was little difference in biological repetition among different groups of sequencing data,and the experiment was repeatable.2.There is crosstalk between Wnt signaling pathway and Neurotrophin signaling pathway,and the interaction factors Wnt4?FZD6?Ctnnb1? Igf1?Igf1r?Ngf and Ntrk3 may be the key target genes.3.Ctnnb1 may be the key regulator in the process of glial cell transdifferentiation into neurons.