Differential Induction Of Bovine Muscle Satellite Cells Into Pancreatic Cells

Muscle satellite cells are potential myogenic cells capable of self-renewal and with potential multi-directional differentiation. Under normal circumstances these cells are in resting state, but they can proliferate, differentiate and integrate into myotubes and form muscle cells. In this study, the muscle satellite cells were cultured and established from bovine fetal muscle tissues. Then the cells were induced to differentiate into pancreatic cells which were identified by immunohistochemical, quantitative real-time PCR and ELISA analyses. During pancreatic induction process the genes associated with pancreatic development such as PDX1, NGN3, elastase, amylase and INS were analyzed. PDX1, an important transcription factor in the development and differentiation of pancreatic islet, started to express at Day2after induction, and reached to the highest level at Day3; NGN3which is a important transcription factor for the formation of endocrine cells started to express at Day2, and reached to the highest level at Day3; Both elastase and amylase are pancreatic extracellular secretion associated genes. The mRNA expression of elastase was expressed at and kept to the hughest level at Day2to Day4, and then reached to a second top level at Day6to Day12. Amylase mRNA began to express at Day6and reached to the maximum at Day8. The Insulin gene expressed from Day1and reached to the maximum at Day11-12, and the concentration of the secreated insulin reached to the maximum at Day11-12. In conclusion, the bovine muscle satellite cells can be trans-differentiated into pancreatic cells.