| This dissertation describes four projects: 1) development of a procedure for quantifying multi-step reactions for the immobilization of protein on gold and silica surfaces; 2) demonstration that observation of three-regime kinetics of formation of polymer brushes by means of the grafting-to approach is dependent on grafting conditions; 3) development of a sensor that can be used for quantitative detection of Bisphenol A (BPA) in aqueous media; 4) development of a chondroitin sulfate-based synthetic hydrogel to mimic the mechanical properties of the nucleus pulposus (in human intervertebral discs). The first three above were projects in surface modification, and for these, the quartz crystal microbalance played a key role. The fourth project was synthesis of bulk polymers and for this, dynamic mechanical analysis played a key role.;The existence of quantitative analysis methods for the immobilization of proteins is relevant, because proteins are used increasingly in immobilized form for research on their interactions with various ligands. The understanding of three-regime kinetics is important, because of its role in surface tailoring and the potential it offers for creating multifunctional surfaces. A sensor that measures the amount of BPA in aqueous media is relevant, because surface waters are still polluted with this endocrine-disrupting chemical. Finally, a hydrogel with properties that duplicate those of the human nucleus pulposus is important, because the health care establishment is currently considering implants that replace the nucleus pulposus. |
