Effect of porcine luteinizing hormone on intrafollicular milieu and gene expression in granulosa cells and oocytes in dairy cows | Posted on:2015-12-30 | Degree:M.S | Type:Thesis | University:University of Alberta (Canada) | Candidate:Behrouzi, Amir | Full Text:PDF | GTID:2473390017499155 | Subject:Animal sciences | Abstract/Summary: | | In previous research, the use of porcine luteinizing hormone (pLH) in lieu of gonadotropin-releasing hormone (GnRH) for synchronizing ovulation in a fixedtimed artificial insemination protocol in dairy cows improved pregnancy rates (42 vs 28%) without increasing progesterone concentrations. The mechanisms associated with this increase in pregnancy rates are not known. We hypothesized that exposure to pLH alters intrafollicular protein/gene expression compared to GnRH treatment, particularly the oocyte-expressed members of the TGF-beta superfamily, and EGF-like growth factor, which regulate oocyte competence. To address this, two studies were conducted to determine whether replacing pLH with GnRH to synchronize ovulation in dairy cows altered intrafollicular milieu and gene expression in mural granulosa cells and oocyte cumulus complexes. The results indicated that the relative abundance of BMP-15, GDF-9 and TGF-beta1 were greatly increased in pLH-treated cows. Since these factors promote cumulus expansion and oocyte competence, they may have contributed to the pregnancy increase previously reported. | Keywords/Search Tags: | Hormone, Cows, Oocyte, Intrafollicular, Expression, Dairy | | Related items |
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