| Nipah virus (NiV) is an emerging virus in the Paramyxovirus family that causes encephalitis in humans and respiratory disease in pigs. Like all viruses, as intracellular parasites, NiV depends on host cell architecture and molecular processes to replicate. Through systematic analysis, we defined three host factors that associate with NiV-M and are involved in viral replication. Our study used affinity purified mass spectrometry (APMS) to create a virus-host protein interactome. We created a siRNA library to validate the interactions found in the AP-MS study. We identified previously uncharacterized host factors for replication, including VprBP, hnRNP C, and WDR18. Finally, we examined the effects of a VprBP-targeting small molecule compound on NiV infection. The data support the hypothesis that an AP-MS targeted siRNA screen is a powerful tool for identifying potential targets for drug and therapeutic development and provides a better understanding of the interaction between NiV and its host. |
