The work presented in this thesis describes the structural characterization of arginase from rat liver. Each individual excretes ;The 2.1 A resolution crystal structure of trimeric rat liver arginase reveals that this unique metal cluster resides at the bottom of a 15 A-deep active site cleft. Analysis of the structure suggests that arginine hydrolysis is achieved by a metal-activated hydroxide ion that symmetrically bridges the two Mn...
