| Because the role of mutation in the development of cancer is widely accepted (Lipkin et al., 1999; Go et al., 2001; Setlow, 2001), measurements of mutations are more practical than studies of carcinogenesis. Cancer studies are very expensive, require a lot of mice, and take a very long time.{09}In our search for novel methods of cancer prevention, we investigated the anti-carcinogenic properties of dietary restriction and the carcinogenic effects of a Western diet.; In our studies, there was no significant difference in ENU-induced or spontaneous mutant frequencies in the small intestine. There was a difference in eating patterns between the ad libitum and the dietarily restricted mice, where the dietarily restricted mice ate their food all at once whereas the ad libitum-fed mice ate their food sporadically throughout the day. Nevertheless, mice trained to eat in a 2 to 4 hour period did not show any significant difference in spontaneous mutant frequency. No association was found between dietary restriction and mutation rate.; The Western diet administered during development and adulthood resulted in no significant difference in spontaneous mutant frequency. (Abstract shortened by UMI.)... |
