Estudio de herencia y busqueda de un marcador molecular para la resistencia a la deformacion de vainas causada por el virus del mosaico dorado amarillo de la habichuela (Spanish text, Phaseolus vulgaris)

The inheritance of resistance to pod deformation caused by Bean Golden Yellow Mosaic Virus (BGYMV) was studied in two F₂ populations. A population was developed from a cross between the susceptible line PR9556-158, and the resistant line PR 9556-171. A second population was developed from a cross between the susceptible line PR9556-158 and DOR 482, which is the original source of resistance to pod deformation. Field experiments were conducted at the Isabela Substation to evaluate pod deformation on F₂ plants at mid-pod fill at 45 to 65 days after planting. The segregation patterns in both populations were consistent with a single dominant gene conferring resistance to pod deformation. Using Bulk Segregant Analysis, resistant and susceptible F6 lines from the cross (PR9556-158 x PR9556-171) were screened for a molecular marker linked with the putative resistance gene, Bgp. A total of 620 RAPD's primers were evaluated and only one, OP N4, amplified polymorphous bands between resistant and susceptible lines. The linkage of the 450 by band was calculated using the EM algorithm for a repulsion model in a F₂ population. A recombination fraction of 0.41 was obtained, which suggests a weak linkage between the molecular marker and the gene for the resistance. The SW-12 marker for a QTL for BGYMV resistance and the SCAR SR2 for the bgm-1 gene for BGYMV resistance were not associated with resistance to pod deformation.