| This thesis documents the progress that has been made in the field of unconventional computing based on molecular and biomolecular systems. Previously, the progress made in the field, had fostered the ability to recreate many systems that are able to mimic their electronic counterparts by performing Boolean logic operations. Although the novelty and ingenuity needed to develop homogeneous systems cannot be understated, the ability to organize enzymatic systems with the use of a network of flow cells in which each modified with one enzyme that biocatalyzed one chemical reaction has permitted the novel mimicking of switchable logic gates as well as specific reversible logic gates that were previously difficult to recreate.;The gates that have been created with the use of this modular enzymatic approach include the reversible Double Feynman Gate, Toffoli Gate, Peres Gate, Switch Gate and Fredkin Gate, as well as the non-reversible, switchable OR, NXOR, and NAND systems, the Half-Adder, and the Half-Subtractor. Furthermore, the utilization of modular fluidic devices has not only allowed for the realization of these systems, but has also made it possible to bypass many difficulties that hinder traditional biocomputing systems.;In addition to the advantages from the separation of channels and clocking, the use of modular fluidic devices also allows data signals to be routed between different Output channels. In the addition to the straightforward mimicking of the Boolean logic operations that utilize these modular fluidic systems, there is also the ability to integrate these systems into complex biomolecular networks, as well as sense-and-act/logically controlled release systems. Such abilities will give future unconventional biocomputingand bioanalytical systems added benefits leading to a degree of autonomy that is needed for truly theranostic systems. |
