| Carbohydrates are ubiquitous in biological systems. With the advance of coupling techniques, protecting groups and reagents have made the synthesis of complex oligosaccharides become a more manageable task. This provided a valuable tool for biologist and chemist to study carbohydrate functions.; Cancer cells can be distinguished from normal cells by the appearance of large and unusual oligosaccharides on their cell surface. Some of these oligosaccharides have been characterized as a turner-associated antigen. Globo H, which was found on several different types of human cancer, had been characterized as a turner-associated antigen. A new synthetic method of Globo H and its derivatives by using the reactivity-based one-pot synthesis strategy was discussed. Their abilities of binding to antibody MBr1 were probed by using microarray on the glass slide.; HIV-1 (Human Immunodeficiency Virus Type 1) is the causative agent of Acquired Immunodeficiency Syndrome (AIDS). The HIV-1 envelope protein gp120 is highly glycosylated. Man9GlcNAc2 is found expressed excessively gp120. The antibody 2G12, which was able to broadly neutralizing HIV, was found specially recognized Manalpha1-2Man structure. Based on the information, a several Manalpha1-2Man-containing oligosaccharides were designed and synthesized. Their abilities of binding to 2G12 was studied by using ELISA in the microtiter plates. According the results, compound 67 was chosen to couple with the carrier protein. These sugar-protein conjugates were studied their binding affinity to 2G12 by the ELISA assay. |
