Fast Detection of Low Volumes of Mouse Antibodies Using a Nanofluidic Bioarray Chip

We have developed a novel nanobioarray (NBA) chip for the detection of antibodies (Abs) secreted from mouse immune cells. The NBA chip was constructed by sealing PDMS channel plates with a glass slide, and the intersection approach employed to develop various bioarrays whereby multiple samples each react with multiple probes using a simultaneous liquid delivery method for sample introduction. In our system, we utilized several Ab samples to react with multiple peptide probes. We were able to use the NBA chip to detect samples with a solution volume of 500 nL and of a concentration of 0.05 nM which was lower than what was detected using the conventional ELISA method. This device, when used with human samples, has applications in the detection of antibodies secreted from single immune cells and in the diagnosis of diseases.;Keywords: nanobioarray; polydimethylsiloxane (PDMS); peptide probes; antibodies; antigens; enzyme-linked immunosorbent assay (ELISA); intersection approach; disease diagnosis.