Chamber-specific regulation of cardiac myosin, gene, and protein expression in the heart of hibernating grizzly bears, Ursus arctos horribilis | Posted on:2012-01-19 | Degree:M.S | Type:Thesis | University:California State University, Long Beach | Candidate:Barrows, Nathan D | Full Text:PDF | GTID:2454390008491728 | Subject:Biology | Abstract/Summary: | | Grizzly bears, Ursus arctos horribilis, tolerate extended periods of extremely low heart rate during hibernation without developing congestive heart failure or chamber dilation. I hypothesized that relative MyHC isoform ratio would be unchanged during hibernation to maintain contractility during hibernation-associated bradycardia. Myosin heavy-chain (MyHC) proteins are the primary determinates of contractility, and I report a novel shift in expression toward MyHC-alpha isoform in the left atrium during hibernation. Interestingly, mRNA expression of atrogenes and their regulatory transcription factors is not increased in concert with ventricular atrophy, although myocardial IGF-I gene expression is decreased. Proteomic analysis of seasonal protein profiles from left atria and ventricles revealed no significant differences between groups. These findings highlight chamber-specific regulation of relative MyHC expression that may serve to optimize contractility during the altered hemodynamic state of hibernation, and suggest posttranscriptional regulation of cardiac atrophy in hibernating grizzly bears. | Keywords/Search Tags: | Bears, Regulation, Heart, Hibernation, Expression | | Related items |
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