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Survey of Flavonoids and Their Distribution in Different Kinds of Onions Using High Performance Liquid Chromatography and Gas Chromatography -- Mass Spectrometry

Posted on:2012-08-28Degree:M.SType:Thesis
University:East Tennessee State UniversityCandidate:Racharla, ArchanaFull Text:PDF
GTID:2451390008999924Subject:Chemistry
Abstract/Summary:PDF Full Text Request
This research is done to determine the distribution of flavonoids in diverse varieties of onions (commercial and wild onions). Reflux extraction method was done on dried onions and the abstracts were analyzed subsequently by high performance liquid chromatography (HPLC) and gas chromatography -- mass spectrometry (GCMS). Statistical calculations were done to see if there are significant differences in the varieties of onions studied from the chromatographic profiles obtained. The chromatograms obtained were patterned using visual observations, scatter plot study, correlation co-efficient, and ANOVA to evaluate the significant difference of the distribution of flavonoids in varieties of onions. The organic white onions seem to have closer flavonoids profile to that of natural wild onions with a correlation co-efficient of 0.99 from HPLC data and 0.88 from the GCMS data. The ANOVA results also support these conclusions. However, natural wild onions tend to have more constituents that can be beneficial.
Keywords/Search Tags:Onions, Flavonoids, Distribution, Chromatography
PDF Full Text Request
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