| A perfusion bioreactor allowing continuous feeding and extraction of various metabolites has been developed and validated for plant cell cultures. A cell free culture medium has been recirculated through an external resin extraction column to adsorb secondary metabolites. The objective was to adapt the flask system developed by Klvana et al. (2004) to bioreactor. Cell suspensions of Eschscholtzia californica (california poppy) have been used as model system for continuous production of benzophenanthridin alkaloids. Based on sedimentation, the system I developed was composed of four cell/medium separation columns mounted inside a 3-L bioreactor in order to separate cells and aggregates from the culture medium while imposing low shear stress on the cells. The culture medium was recirculated through an external circulation loop containing three XAD-7 resin columns, used one after the other. The columns were filled with for adsorption of alkaloids. (Abstract shortened by UMI.)... |
