| The auxin-resistant4 (axr4) mutants of Arabidopsis thaliana are characterized by auxin-resistant roots with reduced lateral root growth and gravitropic response. The auxin influx carrier, AUX1 protein, is mislocalized in axr4 mutants, which means that the AXR4 protein is necessary for proper AUX1 localization. The exact function of AXR4 remains unknown. To determine the function of axr4, two enhancers of the axr4-3 mutant's auxin resistance were studied in this research. The enhancers were found not to enhance axr4-3 mutant phenotypes in morphology, hypocotyl elongation, abscisic acid resistant root and lateral root growth, and auxin uptake. The WM20.2 enhancer was also mapped in this research. Further characterization and mapping can finally find the gene affected in these enhancers and provide a better understanding of AXR4 and auxin transport. |
