Exploiting micro-scale magnetic and acoustic phenomena in microfluidic devices for cell sorting applications | Posted on:2011-11-13 | Degree:Ph.D | Type:Thesis | University:University of California, Santa Barbara | Candidate:Adams, Jonathan D | Full Text:PDF | GTID:2444390002961821 | Subject:Physics | Abstract/Summary: | | The capability to sort specific biological targets from complex mixtures is critical for many biomedical applications, ranging from in vitro diagnostics to cell-based therapies. As these applications expand and demands on performance increase, a need has arisen for new technologies that are capable of high-purity, high-throughput separation. The use of microfluidics and micro-systems technology is a compelling approach to meet this demand. In this thesis, we present a series of microfluidic devices that exploit sub-mm-lengthscale phenomena in magnetics and acoustics for high-performance cell and particle separation.;First, we show an architecture to enable separation of multiple magnetic targets in a simultaneous manner. We demonstrate the capability to simultaneously sort multiple multiple bacterial cell types with > 90% purity and > 500-fold enrichment at a throughput of 109 cells/h.;We next report the use of ultrasonic standing waves to achieve cell cycle phase synchronization in mammalian cells in a high-throughput and reagent-free manner. We show that ultrasonic separation allows for gentle, scalable and label-free synchronization with high G1-phase synchrony (≈ 84%) and throughput (3 x 106 cells/h per microchannel).;Third, we discuss the integration of microfluidic acoutic and magnetic separation in a monolithic device for multi-parameter particle separation. Using our integrated device, we demonstrate high-purity separation of a multicomponent particle mixture at a throughput of up to 108 particles/h.;Subsequently, we describe a tunable acoustophoretic separation architecture capable of sorting cells and particles based on a range of sizes, analogous to a band-pass filter. We show that our device is capable of sorting an arbitrary particle size range between 3 and 10 mum in diameter with high efficiency (transfer fraction = 0.98 +/- 0.02) at a throughput of ≈ 10 8 particles/h per microchannel.;Finally, we discuss our ongoing efforts to develop a new device architecture for acoustic separation capable of greatly increased throughput. We demonstrate preliminary results showing separation of blood components at whole blood concentrations at a sample flow rate of about 1 L/h. | Keywords/Search Tags: | Separation, Cell, Device, Magnetic, Microfluidic, Sorting | | Related items |
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