| The auxin resistant 4 (axr4) mutant is recessive and causes plants to be auxin resistant. axr4-2 impedes auxin transport by altering the localization of the auxin importer, AUX1. In this project, an enhancer mutation was identified in order to further understand the function of AXR4. The FE37.5.1 enhancer mutation has been identified to be auxin sensitive recessive. The double axr4-2 + enhancer mutant have been found to be more auxin resistant than axr4-2 when comparing primary roots. The enhancer mutation was isolated and it did not produce an enhanced phenotypic response for lateral root formation and gravitropic response. This may suggest that the enhancer mutation may disrupt in some pathway that is different from how axr4-2 and axr4-2 + enhancer respond to root growth. The enhancer mutation itself has been identified to a region on chromosome 5, but the current region is too broad to narrow down. |
