Study Of The Extraction, Separation And Purification Of The Saeweed Auxin

Taking indole-3-acetic acid ( IAA) as an example of auxin , the methods for extracting , The method of extracting the auxin with organic solvent , separating and purifying the auxin by aether extraction method and column chromatography has been established. According as GC method to determine the nature determine auxin This study of the experiment acquires of the result and data is as follows :1,The petroleum ether decolor process, with the petroleum ether under the weak alkali term taking off the color of IAA,losing efficiency is lowest .2,Aether extracting effect is better than ethyl acetate.Under PH3.0 Aether extracting effect is best.3,Make sure the pH=6.0, 0.05mol/LNaHPO4-citric acid buffer liquid as eluted liquid of column chromatography.4,GC method examines,peak value time of experiment sample is alike same with standard sample and , really can say ,experiment sample is auxin—IAA.5,The ercent of the auxin is 5.24%, the contain of the undaria pinnatifida is 7.70%.