Role of collagen, complement C3, and C5 on cutaneous wound healing: Topical formulation, preparation, and in-vivo evaluation in experimental rat

The growing rates of problematic wounds in the population and the subsequent increase in morbidity and mortality warrant further understanding of wound healing and the development of therapeutic agents targeted to alleviate these devastating concerns. The complement system is composed of bactericidal and hemolytic proteins that increase capillary leakage and inflammatory cell migration. It allows for an anaphylactic reaction and the recruitment of inflammatory cells. Fibroblast recruitment and subsequent collagen deposition in wounds is responsible for wound healing and is regulated by inflammatory cells. However, little is known about role the complement system may have on wound healing strength. This work investigates the effects of the topical application of collagen, complements C3 and C5 in varied formulations on the paired surgical skin incision rat model. These potential findings may help further enhance the understanding of wound healing and allow for a novel therapeutic approach for the treatment of patients.