| The AFM is an important tool for biological applications because of its great ability to measure three dimensional images with high nanometer scale resolution in a variety of applications. The AFM has been used to image individual biological molecules such as DNA, amino acids, or macro molecules such as protein, entire cells as well as their components and viruses. In some cases, it is possible to watch biological processes in real time, such as viral attacks on cells, without harming live biological samples (Rugar et al., 1990, Kuznetsov et al., 2004).;The characteristics and disease pathogenesis of Respiratory syncytial virus (RSV) needs to be understood for the proper development of therapies and vaccines. AFM, which is capable of real time investigation and minimization of damage to live or dead cells, has been used to characterize and investigate of HEp2 cell's membrane and nucleus damage caused by RSV. As RSV exposure time is increased, the overall step height of the cell membrane and nucleus were decreased but some materials inside of nucleus, presume the nucleolus, remained. It is also possible that the presence of the Respiratory syncytial virus (RSV) might be observed on the nucleus of virus infected HEp2 cells. (Abstract shortened by UMI.)... |
