The Effect Of Yitangkang On JAK/STAT Signaling Pathway In Kidney Tissue Of Diabetic Rats With Deficiency Of Both Qi And Yin

Purpose: In this experiment,we explored the effect of Chinese herbal compound Yitangkang on the expression of jak2,stat3 gene and protein and its phosphorylation in jak/stat signaling pathway of qi and yin deficiency type diabetic rats,and further explored Yitangkang against qi and yin deficiency type diabetes.The mechanism of action of rat kidney protection.Material and method: In this experiment,70 male Wistar male rats of 8 weeks old were selected.After one week of adaptive feeding,they were divided into blank group 10 and Type 2 diabetes group by random number table.Type 2 diabetes mellitus rats were given intraperitoneal injection of 45 mg/kg stz solution to achieve Type 2 diabetes.Standard?random blood glucose values are ?16.7 Mold/l?;The model rats were divided into 10 control groups and 40 Qi and Yin deficiency groups according to the random number table method.The model of TCM and yin deficiency was established in the model of Qiyin deficiency and deficiency.The rats in this group were given 15.75g/?k·d?of gas-consuming yin granules.After 4 weeks,the rats developed soft stools.The fur is slightly less,the dull dark yellow,the hair is erect,the arch back,the skin is slack,the spirit is weak,the activity is reduced,the movement is lazy,and a few are irritable,the tongue is fat,the tongue is red or the tongue is dim,smooth and no Moss or moss white or greasy performance,that is to achieve the standard of Qi and Yin deficiency.After modeling,press random number The table was divided into 10 model group,10 Yitangkang group,10 metformin group,and 10 combined group?Yitangkang + metformin group?.The three drug intervention groups calculated the dosage according to the body surface area replacement algorithm.The stomach was administered once a day for 6 weeks.Blank group feeding during the whole experiment Feed,the other groups continue to feed high-fat and high-sugar feed,free to drink water.During the experiment,blood was collected from the tail vein of the rats every weekend,and the fasting blood glucose level was measured for recording.After the experiment,the rat abdominal aorta was taken for 5 ml/body,and the serum was taken after centrifugation,and the samples were sent for examination.Tissue,the right renal cortex is placed in the cryotube,and the liquid nitrogen is rapidly frozen and transferred to-80 °C.Saved in the box.The left renal cortex was placed in a brown 4% paraformaldehyde solution bottle for he staining.The content of tg and tc were determined by colorimetry.The expressions of fins,umalb,insulin and TXB₂ were detected by elisa kit.The expression of mrna gene in Jak2 and Stat3 was detected by fluorescence quantitative PCR.The expression of mrna gene in Jak2 and Stat3 was detected by western blot.Jak2,Stat3,were detected by western blot.p-jak2,p-stat3 protein expression,he staining observation of morphological changes in the kidney.Results: 1.General condition of rats Compared with the blank group,the rats in the model group of dm qi and yin deficiency showed soft and thin stools,slightly less fur,dull dark yellow,hair erect,arched back,sagging skin,lack of energy,reduced activity,lazy Move,and a few are irritable,easy to panic,and fat,Red Shaojin or dull tongue,smooth and no moss or white or moist.Symptoms improved after treatment with Yitangkang group and combined group.2.Rat body weight,kidney weight,insulin resistance,blood sugar and other indicators Compared with the blank group,the expression levels of body weight,blood glucose and insulin resistance in the model group and the control group were significantly increased?P <0.05?,the renal weight index decreased significantly?P <0.05?;compared with the model group,the expression levels of body weight,blood glucose and insulin resistance in the Yitangkang group,metformin group and combination group were significantly lower?P <0.05?,kidney weight The index is significantly higher?P <0.05?;Compared with the group of Yitangkang and metformin,the expression of body weight,blood glucose and insulin resistance in the combined group was significantly decreased?P <0.05?,and the renal weight index was significantly increased?P <0.05?.3.Rat blood lipids,TXB₂,Insulin and other indicators Compared with the blank group,the expression levels of TG,TC,LDL-C,TXB₂,and Insulin in the model group and the control group were significantly increased?P <0.05?;Compared with the model group,the expression levels of TG,TC,LDL-C,TXB₂,and Insulin in the Yitangkang group and the combined group were significantly lower?P < 0.05?.4.Expression of Jak2 and Stat3 genes in rat kidney Compared with the blank group,the expression of Jak2 and Stat3 genes in the control group and the model group was increased P <0.05).Compared with the model group,the expression of the Jak2 and Stat3 genes was decreased in the Yitangkang group,the metformin group,and the combined group?P <0.05?;Compared with metformin group and Yitangkang group,the expression of Jak2 and Stat3 genes in the combined group was decreased?P <0.05?.5.Expression of Jak2,p-Jak2,Stat3 and p-Stat3 proteins in rat kidney Compared with the blank group,the expression levels of p-Jak2 and p-Stat3 protein in the control group and the model group were increased?P <0.05?.Compared with the model group,the Yitangkang group,metformin group,combined group p-Jak2,p-Stat3 protein The expression level decreased?P <0.05?.Compared with the metformin group,the expression of p-Jak2 and p-Stat3 protein decreased in the combined group?P <0.05?.Compared with the Yitangkang group,the combined expression of p-Stat3 protein decrease?P <0.05?.Conclusion: 1.Chinese herbal compound Yitangkang can improve the body weight and kidney weight of dm model rats,improve insulin resistance,lower blood sugar,reduce kidney tissue damage,and play a role in kidney protection.The combined effect of the combination group is more significant.2.Chinese medicine compound Yitangkang can delay the progression of diabetic kidney injury and repair damaged kidney group Its therapeutic mechanism and intervention inhibit the phosphorylation of Jak2 and Stat3,thereby inhibiting the expression of Jak2/S tat3 signaling pathway and suppressing the development of dm renal inflammation.This may be one of the mechanisms of Yitangkang's protective function on dm kidney function.