Pharmaceutical Research Based On The Traditional Chinese Medicine "mask" For Atmospheric PM2.5 Protection And Replenishing Qi Ointment

Purpose:Based on current status that atmospheric PM2.5 pollution causes many diseases such as respiratory system and cardiovascular system.In accordance with the traditional thinking of"preventive treatment of disease",through the preliminary pharmaceutical research of porous chitosan membrane with drug and Buzheng Yiqi oral thick paste,the external protections of traditional Chinese medicine membrane such as filtration,bacteriostatic and the method of nourishing and invigorating qi by taking traditional Chinese medicine oral thick paste were used to jointly play the role of resisting exogenous pathogens.Using this solution to provide new ideas and new preparations for the internal and external treatment of Chinese medicine to protect against atmospheric pollution.Material and Method:1.The research of preparation technology test.The experiment was carried out by single factor analysis method.Taking the extraction amount of volatile oil as the evaluation index to optimize the extraction method of volatile oil from Largehead Atractylodes Rhizome,Divaricata Saposhnikovia and Manchurian Wildginger Herb;The experiment was carried out by L₉?3⁴?orthogonal test design.Taking the total polysaccharide extraction amount,the creaming rate,the total extraction amount of astaxantin and schisandra alcohol as comprehensive evaluation indexes to optimize the water decoction process of Mongolian Milkvetch Root and Schisandrae Chinensis Fructus;According to the edition of PRC?2015?to screen the auxiliary materials and their dosage.Taking the relative density of clear paste as the evaluation index to optimize the concentration process and the preparation process of Buzheng Yiqi oral thick paste.Using chitosan acetic acid solution as the raw material of the membran.Taking the film molding property,pore size and distribution as evaluation indexes to screen the concentration and dosage of chitosan acetic acid solution,the type and dosage of pore-forming agent and the method of adding volatile oil,and then to optimize the preparation conditions of porous chitosan with drug by this way.2.The research of quality standard test.The qualitative identification of the traditional Chinese medicines of Mongolian Milkvetch Root,Divaricate Saposhnikovia and Schisandrae Chinensis Fructus in Buzheng Yiqi oral thick paste.According to the edition of PRC?2015?to examine the appearance,relative density and insolubility of Buzheng Yiqi oral thick paste.To determine the content of Cimicin and Schisandrin A in Buzheng Yiqi oral thick paste by HPLC.The qualitative identification of the traditional Chinese medicines of Largehead Atractylodes Rhizome and Manchurian Wildginger Herb in porous chitosan membrane with drug.According to the edition of PRC?2015?to examine the appearance and difference of porous chitosan membrane with drug.To determine the content of methyleugeno in porous chitosan membrane with drug by HPLC.3.Antibacterial test of microporous chitosan membrane.Using K-B method to determine the antibacterial effect of the porous chitosan membrane on Staphylococcus aureus,Klebsiella pneumoniae,Pseudomonas aeruginosa,Streptococcus pneumoniae,Streptococcus pyogenes and Haemophilus influenzae.Results:1.Preparation technology of Buzheng Yiqi oral thick paste:Taking the prescription amount of Largehead Atractylodes Rhizome,Manchurian Wildginger Herb,Divaricata Saposhnikovia with 8 times water for 0.5h,extracting for 6 hours and seting aside volatile oil,liquid and residue?volatile oil is used to prepare porous chitosan membrane with drug?.The amount of water added to Mongolian Milkvetch Root,Schisandrae Chinensis Fructus is 12,10 and 10times,extracting 3 times for 2 hours and volatile oil residue is added at the last time.The decoction is reduced in pressure and concentrated to 600g.The prescription amount is added to the refined honey to make 1000g of sachet and taked 20g three times a day;Preparation technology of porous chitosan membrane with drug:Taking 5g 2.5%chitosan acetic acid solution,1%glycerol and 5%uniform pore are added in chitosan acetic acid solution,and coating on the glass membrane.Using 1%NaOH solution of acetone to remove residual solvent.Using water to wash to neutral.Removing pore-causing agent with acetone.Washing with water to make it neutral to make porous chitosan membranes?100mm×70mm?.Adsorption method is used to load the naphtha and drying at 40?.When inserted into the mask nose part interlining,can be used.2.A qualitative identification method for the medicinal materials of Mongolian Milkvetch Root,Schisandrae Chinensis Fructus and Divaricate Saposhnikovia was established in Buzheng Yiqi oral thick paste;The relative density and insolubility of the Buzheng Yiqi oral thick paste complies with the relevant provisions of the edition of the PRC?2015?under the“Ointment”;The HPLC method was used to determine the content of astaxanthin and schisandrin in Buzheng Yiqi oral thick paste.A qualitative identification method for the medicinal materials of Largehead Atractylodes Rhizome,Manchurian Wildginger Herb was established in the porous chitosan membrane with drug;The weight difference of porous chitosan membrane conforms complies with the relevant provisions of the edition of the PRC?2015?under the“Ointment”;The HPLC method was used to determine the content of methyleugeno in porous chitosan membrane.3.According to the corresponding positive drug sensitivity standard,the antimicrobial effect of the porous chitosan membrane with drug on Pseudomonas aeruginosa and Klebsiella pneumoniae was rated as"I"which is moderately sensitive.With certain antimicrobial effect which can be used to protect the respiratory tract bacterial infection caused by Pseudomonas aeruginosa and Klebsiella pneumoniae.Conclution:This paper determines the optimal preparation conditions for the Buzheng Yiqi oral thick paste and porous chitosan membrane with drug.Established Buzheng Yiqi oral thick paste,porous chitosan membrane with drug system and stable quality standards.The microporous chitosan film was determined to have a certain antibacterial effect against Pseudomonas aeruginosa and Klebsiella pneumoniae.Providing reference and theoretical basis for the future research and development of the preparation.