| Objectives: The reparation of the dentin-pulp complex after injury depends on tertiary dentinogenesis to reconstitute normal tissue continuum at the pulp-dentin border. It has been observed that transforming growth factor-beta1 (TGF-β1 is deeply involved and plays a positive role in this process, which provides the basis for developing biomaterials as a promising strategy for a successful regenerative pulp treatment. In this study, we attempted (1) to develop asymmetric porous chitosan membranes in combination with TGF-β1-loaded chitosan microspheres (MS-TGFs), (2) to evaluate the effect of released TGF-pi on the proliferation potential of odontoblast cell line MDPC-23 in such membranes, (3) to investigate the dentinogenic effect of the material as direct pulp capping material on mechanically exposed pulps in dog molar and canine teeth. Methods: (1) Chitosan microspheres were produced by an emulsion-crosslinking method, with TGF-β1 loaded into them by adsorption. The controlled release of TGF-β1, as measured by enzyme-linked immunosorbent assay (ELISA), was... |
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