Pyrosequencing Technology Explores The Relationship Between Gene Polymorphisms Of Children's Purpura Nephritis And TCM Syndrome Types

Purpose: To investigate the correlation between MUTYH,PIK3C2 B,ALDH1L1,TLR1,MAML3,PLG,COMP,AGT gene polymorphisms and clinical classification and TCM syndromes in children with purpuric nephritis.Material and method:The subjects were composed of 49 children with HSPN and 21 healthy children.DNA was extracted from peripheral venous blood,and after PCR amplification,MYTYH,PIK3C2 B,ALDH1L1,TLR1,MAML3 were obtained by pyrosequencing(Pyrosequencing).PLG,COMP,and AGT genes were tested for single nucleotide polymorphisms.The genetic susceptibility of HSPN was explored by analyzing the genotypes of the Western medicine and Chinese medicine groups and their allele frequencies.Apply SPSS23.0 for windows software for statistical analysis.Results:1.By analyzing HSPN children and healthy children,it was found that there were low frequency mutations in the following gene loci,but it was not statistically significant:(1)MUTYH gene rs35352891 locus,HSPN children and healthy children were not significantly different(P =0.736,OR = 2.474,95% CI = 0.325-18.856,P>0.05)(2)There was no significant difference in PIK3C2 B gene rs17847749 between children with HSPN and healthy children(P = 1.000).(3)There was no significant difference in children with HSPN between rsHHL1L1 gene and rs140551047.(P = 1.000)(4)There was no significant difference in children with HSPN between TLR1 gene rs200212492 and healthy children(P = 1.000).(5)There was no significant difference in the HSML3 gene rs534986978 HSPN children compared with healthy children.(P = 0.549,P>0.05)(6)There were no differences between children with HSPN of rs4252128 and rs747448179 atPLG gene and healthy children.Statistical significance(P = 1.000)(7)There was no significant difference in children with HS176 rs4762 locus AGT from healthy children(P = 0.728,OR = 1.875,95% CI = 0.381-9.228,P>0.05).2.By comparing HSPN children with healthy children,the following gene loci were found to be free of mutations:(1)ALDH1L1 gene rs778022672 locus(2)MAML3 gene rs570805279 locus(3)COMP gene rs566112479 locus3.AGT gene rs699 site HSPN children have genetic susceptibility compared with healthy children:(1)genotype CC distribution was statistically significant.(P = 0.037,OR = 3.046,95% CI = 1.049-8.842,P<0.05)(2)Allele C frequency is significantly higher than healthy children.(P = 0.063,OR = 2.32,95% CI = 0.941-5.719,P greater than 0.05)4.Statistical results of AGT gene rs699 locus polymorphism and TCM syndrome types:(1)The difference between the heat-toxic injury syndrome group and the healthy group is close to statistical significance.(P = 0.055,OR = 2.842,95% CI = 0.960-8.410,P > 0.05)(2)There was no significant difference between the Qi deficiency and blood stasis syndrome and the healthy group.(P = 0.277,OR = 5.5,95% CI = 0.545-55.494,P > 0.05)Conclusion:1.rs35352891 locus,rs17847749 locus,rs140551047 locus,rs200212492 locus,rs534986978 locus,rs4252128,rs747448179 locus,rs4762 locus in children with purpuric nephritis low frequency mutation,the results were not statistically significant.2.rs778022672 locus,rs570805279 locus,rs566112479 locus in children with purpuric nephritis without mutations.3.rs699 locus gene CC type P = 0.037,OR = 3.046,95% CI = 1.049-8.842,P <0.05,statistically significant.It has genetic susceptibility in children with purpuric nephritis.4.In the children with HSPN with heat-tolerant injury syndrome,the rs699 locus gene CC type P = 0.055,OR = 2.842,95% CI = 0.960-8.410,P?0.05,close to statistical significance."Hot toxicity" may be an important factor influencing the onset of HSPN in children.