Study On The Effect Of Musk-Boswellia On The Expression Of Protein Kinase-C And Protein Kinase-A In Mice With Chronic Non-bacterial Prostatitis

Objective: to establish chronic nonbacterial prostatitis(CNP)model of NOD mice and investigate the therapeutic effect of musk,frankincense,knotweed and combined application on CNP of NOD mice and the effect on the expression levels of PKC and PKA,the key phosphorylation pathways of tightly coupled protein.Methods: 54 male NOD mice were randomly divided into 9 groups: Mu group,Ma group,Mu-Ma group,Kn group,Mu-Ma-Kn group,high-dose Mu-Ma group,celecoxib group,model group and blank control group.Except the blank control group,were immunologically induced by subcutaneous injection of SD rats' prostate protein purified liquid,and the CNP model was established.After 21 days of antigen injection,the blank control group and model group were given the same amount of normal saline gavage.After 14 days of continuous intragastric administration,the prostate tissues were dissected and isolated,and the pathological changes of the prostate tissues were observed by HE staining.The expressions of PKC and PKA in prostate tissues were detected by WB and fluorescence immunoassay.Through comprehensive evaluation of effect indicators,the therapeutic effect of Mu,Ma,polygonum cuspidatum and combination of drugs on CNP in NOD mice and the effect on the expression levels of Claudins protein phosphorylation key pathways PKC and PKA were studied.Results: 1.Compared with NOD mice in the normal group,the pathological manifestations of prostate tissues in NOD mice and normal mice in the model group were significantly different,indicating that the model was successful.Significant pathological changes were observed in the prostate tissues of both the experimental group and the celecoxib group.The inflammatory infiltration degree of each group ranged from high to low: model group,polygonum cuspidate group,Mu group or Ma group,Mu-Ma group,high-dose Mu-Ma group,Mu-Ma group,celecoxib group,blank group.There was no significant difference in PKA and PKC expression levels between Mu group and Ma group(p> 0.05),and no significant difference between Mu-Ma and celecoxib group(p>0.05).PKA expression level from high to low: model group>Kn group>Mu group or Ma group>Mu-Ma group>high-dose Mu-Ma group>blank group(p<0.01).PKC expression level was from low to high: model group <Kn group<Mu group or Ma group<Mu-Ma group<high-dose Mu-Ma group or celecoxib group < blank group(p<0.05).Conclusion: 1.The pathological state of CNP,PKA expression levels rise,PKC expression level,indicate prostate epithelial permeability barrier system declines,Mu,Ma alone can make PKA expression level cut,PKC expression levels rise,Mu-Ma "should be" can be enhanced when using the trend,so as to increase the permeability barrier function of the prostate;2.Mu-Ma with high dose has a more obvious regulatory effect on prostate barrier permeability;3.The application of Mu-Ma can enhance the therapeutic effect of Kn on chronic non-bacterial prostatitis.