The RNA Binding Protein UNR Promotes The Migration Of Human Glioma Cells And Regulates The Expression Of RPL9

RNA-binding proteins have extensive roles in the regulation of gene expression and have been identified as modulators of cancer development and progression.UNR(Upstream-of-N-Ras),also known as CSDE1(cold shock domain containing E1),is a kind of RNA-binding protein that possesses five conserved cold shock domains and mainly regulates mRNA translation and stability in cell physiological processes.Recently,some studies have shown that UNR promotes the invasion and metastasis of human melanoma and it has been considered a potential prognostic biomarker for pancreatic ductal adenocarcinoma.However,whether UNR participates in the development of glioma is poorly reported.In our study,we first discover that UNR has a high expression level in glioma cell lines and patient tissues.Further,knockdown of UNR characteristically inhibits glioma cell migration but does not inhibit cell proliferation.Moreover,according to the sequencing data of UNR iCLIP-seq,RNA sequencing and ribosome profiling databases of human melanoma,we verify that UNR can also bind to the 3'UTR of PTEN and ribosomal protein L9(RPL9)in glioma cell lines by RNA immunoprecipitation and biotin pull-down assays.We also discover that knockdown of UNR inhibits the expression of RPL9.The EMT-related marker Vimentin is positively regulated by RPL9.Here,we discover the role of UNR in glioma cell lines and establish a link between UNR and RPL9 protein,which will provide new ideas for the further study of glioma.