Panax Notoginsenoside R1 Protects The Small Intestine After Ischemia-reperfusion And Its Relationship With NGF And ProNGF Signaling System

Objectives To investigate the protective effect of notoginsenoside R1 on small intestinal ischemia-reperfusion(I/R)injury and its relationship with NGF signaling pathways(TrkA)and proNGF signaling pathways(proNGF,sortilin,p75).Methods The small intestine I/R model was prepared by clamping the SD rat male mesenteric upper artery for 30 min and then returning the blood supply,and divided into normal group(without any treatment),ischemia-reperfusion group(recovery after clamping),control group(clogging recovery after feeding the tail vein injection of notoginsenoside R1,divided into 1mg/kg,5mg/kg,10mg/kg),saline group(clogging recovery after feeding the tail vein injection of lml/kg saline).The time of the experiment was restored 12h,24h,48h and 72h after blood supply.Pathological examination was performed by HE staining and the villus height and mucosal thickness were measured.PCR were used to analyze the expression of TrkA,proNGF,sortilin and p75 at the gene level respectively.Results Small intestine I/R injury led to changes in the histopathological morphology of small intestine in rats.From the beginning of 12h with the prolongation of reperfusion time,tissue injury gradually reduced,but after administration of 10mg/kg of notoginsenoside R1 can significantly reduce the I/R injury caused by small intestinal tissue pathological damage;I/R injury resulted in down-regulation of TrkA gene expression in the small intestine,on the other hand,proNGF,sortilin and p75 gene expression up-regulation.The expression of TrkA,proNGF,sortilin and p75 after the use of 10mg/kg of notoginsenoside R1 was reversed.Conclusions Notoginsenoside R1 has a protective effect on small intestine I/R injury in SD rats,and its effect is likely to be achieved by regulating NGF and proNGF signaling systems.